文章摘要
殷霞丽,谢 丽,禹立霞,钱晓萍,刘宝瑞.良恶性胸腹水中游离DNA含量及完整性比较[J].南京医科大学学报,2009,29(4):516~519
良恶性胸腹水中游离DNA含量及完整性比较
Cell-Free DNA and DNA integrity in malignant and benign effusions
投稿时间:2008-11-05  
DOI:10.7655
中文关键词: 胸腹水  DNA 完整性  ALU序列
英文关键词: effusion  DNA integrity  ALU repeats
基金项目:
作者单位
殷霞丽 南京医科大学鼓楼临床医学院肿瘤中心暨南京大学临床肿瘤研究所,江苏 南京 210008 
谢 丽  
禹立霞  
钱晓萍  
刘宝瑞  
摘要点击次数: 1416
全文下载次数: 187
中文摘要:
      目的:探讨细胞游离DNA含量及DNA 完整性在良恶性胸腹水中的临床意义?方法:应用实时ALU-qPCR(115 bp?247 bp)方法分析28例良性胸腹水患者和38例恶性胸腹水患者的胸腹水中细胞游离DNA含量以及DNA完整性的情况?结果:ALU247片段含量在良恶性胸腹水组中有统计学差异 (P < 0.05),但ALU115片段在两组中并无统计学差异(P > 0.05);恶性胸腹水中DNA 完整性明显高于良性对照组(P < 0.05)?DNA 完整性的ROC曲线下面积为0.716(P < 0.01)?结论:恶性胸腹水有较高的ALU247片度含量以及DNA 完整性,其中DNA 完整性可能是一个很有价值的诊断指标?
英文摘要:
      Objective:To investigate the cell-free DNA amount and DNA integrity in benign and malignant effusions. Methods:The samples of twenty-eight benign effusions and thirty-eight malignant effusions were collected and analyzed for the DNA amount and integrity by the real-time ALU-qPCR(115 bp,247 bp). Results:The content of ALU247 fragment is statistically different between two groups(P < 0.05),while there is no statistical difference between the two groups in the content of ALU115. When the DNA integrity was compared, the malignant samples had a relative higher DNA integrity(P < 0.05). AUC of ROC for DNA integrity was 0.716(P < 0.01). Conclusion:Malignant effusion samples had a significantly higher amount of long DNA and DNA integrity, and DNA integrity may be a potential biomarker to distinguish malignant effusions from benign effusions.
查看全文   查看/发表评论  下载PDF阅读器
关闭