胰岛素样生长因子-1对胶质瘤C6细胞垂体瘤转化基因的影响
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江苏省高校自然科学研究课题(05KJD320238)


Effect of insulin-like-growth factor-1 on pituitary tumor transforming gene in C6 cells of glioma
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    摘要:

    目的:探讨胰岛素样生长因子-1(Insulin-like-growth factor-1,IGF-1)在体外对胶质瘤C6细胞垂体瘤转化基因(pituitary tumor transforming gene,PTTG)表达的影响-方法:体外培养胶质瘤C6细胞,给与不同浓度的IGF-1(0.1-1.0-10.0 ng/ml)作用24 h后半定量逆转录聚合酶链式反应(RT-PCR)法检测PTTGmRNA,Western blot检测PTTG蛋白表达-结果:不同浓度IGF-1作用后C6细胞PTTG mRNA表达与蛋白水平均增高,差异有统计学意义(P < 0.01),且随着浓度的增加,其升高越明显-结论:IGF-1可以上调PTTG 的表达,并剂量依赖性-

    Abstract:

    Objective:To investigate the effect of insulin-like-growth factor-1(IGF-1) on expression of pituitary tumor transforming gene(PTTG)in C6 cells of glioma. Methods:Glioma C6 cells were divided into four groups and treated with different concentration of IGF-1 for 24 h respectively(A:0 ng/ml;B:0.1 ng/ml;C:1.0 ng/ml;D:10.0 ng/ml). PTTG mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR),Western blot was used to detecte the expression of PTTG protein. Results:The expressions of PTTG mRNA and protein increased dramaticly in the groups treated with IGF-1,and there was a significantly difference between each each groups(P < 0.01). Conclusion:IGF-1 can upregulate the expression of PTTG significantly in dosage-dependent manner.

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戴如飞,张世明.胰岛素样生长因子-1对胶质瘤C6细胞垂体瘤转化基因的影响[J].南京医科大学学报(自然科学版),2009,29(6):794-796

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  • 收稿日期:2008-12-04
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