Objective:To examin the single nucleotide polymorphism(SNP) in COX-2 promoter(-1195G>A) and the association between pathological parameters and COX-2 expression in colorectal cancer. Methods:Genotyping of the COX-2 promoter -1195G>A was carried out by single-tube SYBR GREEN PCR with Tm-shift primers. 118 samples of colorectal cancer patients were anaylzed by genotyping. Results:Stratified analysis indicated that an elevated risk of lymph node metastasis and advanced stage was observed in subjects who carried at least one A allete(AA or GA) compared with patients with GG genotype(P=0.007,0.010,respectively). High expression of COX-2 was found in subjects carrying AA or GA genotype compared with patients with GG genotype(OR= 3.01and 2.79 respectively). Conclusion:COX-2 SNP-1195G>A was associated with clinicobiological behaviors of tumor, such as lymph node metastasis,tumor stage, and expression of COX-2 protein. -1195A allele indicated high risk of lymph node metastasis,advanced stage and high rate of COX-2 staining positive in immunohistochemisty.