两种不同的染色方法评价大鼠心肌缺血-再灌注损伤程度
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南京医科大学科技发展重点项目(06NMUZ006),南京市卫生局医学科技发展课题资助项目(YKK08137)


Assessment of myocardial ischemia-reperfusion injury with two different staining methods
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    目的:探讨评估心肌缺血-再灌注损伤程度的两种染色方法-方法:大鼠冠状动脉左前降支结扎缺血30 min,再灌注6 h形成缺血-再灌注损伤动物模型-超声检测心功能指标,并分别用TTC单染法和Evans blue-TTC双染法评价心肌的损伤程度-结果:Evans blue-TTC双染法可以评价出心肌的缺血组织面积-梗死组织面积和正常组织面积,而TTC单染法只能测出组织的梗死面积和缺血面积,不能排除由于结扎位置不同而引起的误差-同时,双染法得出的梗死面积/危险面积的比值(I/R)与心功能指标的相关性,比单染法得出的梗死面积/左心室的面积比值(I/T)与心功能指标的相关性更高-结论:Evans blue-TTC双染法比TTC单染法能更客观更准确的反映出心肌组织缺血-再灌注损伤的程度-

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    Objective:To discuss two different ways of staining methods in evaluating myocardial ischemia-reperfusion injury. Methods:Anesthetized SD rats were subjected to myocardial ischemia reperfusion injury(ischemia 30 mins/reperfusion 6 hrs). Hemodynamic parameters were measured upon the completion of reperfusion in rats. The hearts were stained with TTC or Evans blue-TTC double-staining,separately,to determine the extent of myocardial damage. All the rats used in the experiment had the same LV morphology and the similar function. Results:The Evans blue-TTC double-staining method can measure the ratio of infarct areas-to-total left ventricular areas(I/R ratio),While the TTC staining can only measure the ratio of infarct areas-to-risk areas(I/T ratio). Compared with the I/T ratio,the I/R ratio has higher relevance with the cardiac function. Conclusion:The Evans blue-TTC double-staining method can determine the degree of myocardial damage induced by the ischemia-reperfusion injury more objectively and accurately than the TTC staining method.

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杨 帆,赵文来,米琼宇,谢利平,刘 振,张 雯,李晓珍,黄 艳,季 勇.两种不同的染色方法评价大鼠心肌缺血-再灌注损伤程度[J].南京医科大学学报(自然科学版),2009,29(8):1055-1058

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  • 收稿日期:2009-04-06
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