文章摘要
曹剑秋,秦 霞,柏 惠,陈 琪.甘氨酸在神经母细胞瘤细胞ATP耗竭损伤中的保护作用[J].南京医科大学学报,2009,29(8):1063~1066
甘氨酸在神经母细胞瘤细胞ATP耗竭损伤中的保护作用
Cytoprotection of glycine on the ATP-depleted SH-SY5Y cells of neuroblastoma
投稿时间:2009-03-30  
DOI:10.7655
中文关键词: 甘氨酸  ATP耗竭  甘氨酸受体  细胞保护
英文关键词: glycine  ATP depletion  glycine receptor  cytoprotection
基金项目:海外青年学者合作研究基金(30628017)
作者单位
曹剑秋 南京医科大学动脉粥样硬化研究中心,江苏 南京 210029 
秦 霞  
柏 惠  
陈 琪  
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中文摘要:
      目的:观察甘氨酸(glycine,Gly)对ATP耗竭的神经母细胞瘤细胞SH-SY5Y的保护作用及其可能机制?方法:通过乳酸脱氢酶(LDH)释放?四甲基噻唑蓝(MTT)实验及细胞内ATP含量的检测,观察ATP耗竭时Gly对SH-SY5Y细胞的保护作用,通过Western blot检测甘氨酸受体的表达情况?结果:耗竭1 h内,Gly保护组细胞内的ATP含量高于耗竭组?耗竭6 h后,Gly保护组细胞的LDH释放率较耗竭组明显下降,MTT吸光度也明显上升?同时,ATP耗竭后细胞甘氨酸受体表达上调,这种上调趋势在使用甘氨酸后有所缓解?结论:甘氨酸可保护ATP耗竭的SH-SY5Y细胞,并缓解细胞应激时甘氨酸受体的表达上调?
英文摘要:
      Objective:To investigate the cytoprotection and mechanisms of glycine(Gly) on the ATP-depleted SH-SY5Y cells of neuroblastoma. Methods:SH-SY5Y cells were exposed to ATP depletion in the presence or absence of glycine. The cell injury was assessed by measurements of ATP concentration,lactate dehydrogenase (LDH)release and ceu activity. Glycine receptor expression was analyzed by Western blot. Results:Gly was found to ameliorate a reduce in ATP level within 60 minutes of treatment with rotenone and antimycin A. After the treatment for 6 hours,Gly could reduce the LDH release and enhance cell activity. Meanwhile,glycine receptor was overexpressed under the stress,which was decreased by addition of glycine to cells. Conclusion:These results demonstrated that Gly protected SH-SY5Y cells from ATP depletion injury and alleviated over-expression of glycine receptor under stress.
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