文章摘要
冯亚东,郝 波,朱 宏,施瑞华.ERK1/2和Survivin在食管癌中的表达及其相关性[J].南京医科大学学报,2009,29(8):1085~
ERK1/2和Survivin在食管癌中的表达及其相关性
The expression and correlation of ERK1/2 and Survivin in esophageal cancer
投稿时间:2009-03-31  
DOI:10.7655
中文关键词: 食管癌  ERK1/2  Survivin  相关性
英文关键词: esophageal cancer  ERK1/2  Survivin  correlation
基金项目:江苏省科技厅基础研究计划资助项目(BK2005153)
作者单位
冯亚东 南京医科大学第一附属医院消化科,江苏 南京 210029 
郝 波  
朱 宏  
施瑞华  
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中文摘要:
      目的:探讨ERK1/2和Survivin在食管癌中的表达和二者之间的相关性?方法:应用免疫组化法检测40例食管癌和20例正常食管标本中ERK1/2和Survivin的表达?Western blot法检测ERK1/2和Survivin在食管癌中的表达以及PD98059对食管癌Eca109细胞株ERK1/2?Survivin表达的影响?免疫共沉淀法检测ERK1/2和Survivin在食管癌Eca109细胞株中的相互作用?结果:ERK1/2和Survivin在食管癌中表达的阳性率分别87.5%和90.0%,在正常食管组织中表达的阳性率为40.0%和5.0%?Westen blot显示ERK1/2和Survivin在食管癌癌组织中的表达高于癌旁组织中的表达?免疫共沉淀结果提示ERK1/2和Survivin在食管癌中的表达存在相互作用?PD98059下调Survivin的表达?结论:ERK1/2和Survivin在食管癌中高表达,在食管癌Eca109细胞中ERK1/2和Survivin表达有相关性,抑制ERK1/2的表达能下调Survivin的表达?在食管癌中Survivin的表达可能依赖于ERK1/2?
英文摘要:
      Objective:To investigate the expression and correlation of ERK1/2 and Survivin in esophageal cancer. Methods:The expression of ERK1/2 and Survivin on 40 esophageal cancer tissues and 20 normal esophageal tissues were detected by immunohistochemistry. The protein expression of ERK1/2 and Survivin and the consequent changes by PD98059 were detected by Western blot analysis. The correlation of ERK1/2 and Survivin in Eca109 cells was detected by co-immunoprecipitation analysis. Results: Among 40 esophageal cancer tissues,the positive immunolabelling rate of ERK1/2 and Survivin was 87.5% and 90.0% respectively,while among normal esophageal tissues the positive rate was 40.0% and 5.0%. The protein expression levels of ERK1/2 and Survivin in esophageal cancer tissues were higher than those in paraneoplastic tissues. There was coexpression of ERK1/2 and Survivin proteins. PD98059 down-regulated the expression of Survivin in Eca109 cells. Conclusion:The expression of ERK1/2 and Survivin is remarkably up-regulated in esophageal cancer tissues than in paraneoplastic tissues. There is positive correlation between the expression of ERK1/2 and Survivin in Eca109 cells. Inhibition of ERK1/2 by PD98059 induces down-regulation expression of Survivin. The expression of Survivin on esophageal cancers may depend on ERK1/2 expression.
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