objective:to investigate the effects of exogenous wild csk－binding protein(cbp)gene stably transfection on growth of lung adencarcinoma cells in vitro. Methods:at first,a recombinant eukaryotic expression plasmid pcdna3.0－cbp was constructed. Human lung cancer cell line spc－a1 was transfected with pcdna3.0－cbp or mock transfected plasmid pcdna3.0(-)with lipofectamine 2000,and cells that can express cbp stably were screened out by g418(800 mg/l). Change of cbp protein level was measured by western－blot;cell viability was tested by mtt assay. Transwell and wound－healing methods were used to detect the difference of invasion and migration between transfected and non－transfected cells. Results:cbp protein level in pcdna3.0－cbp transfected spc－a1 cells was significantly higher than that in control spc－a1 cells and pcdna3.0 (-)transfected spc－a1 cells. The mtt assay revealed that the cbp gene transfected cells had less proliferation ability than control cells and pcdna3.0(-)transfected spc－a1 cells (p < 0.01). As compared with control cells and pcdna3.0(-)transfected spc－a1 cells,the invasion and migration ability of pcdna3.0－cbp transfected cells decreased obviously(p < 0.01). Conclusion:the cbp gene,as a recently identified transmembrane protein,can restrain malignant phenotypes of the human lung adencarcinoma in vitro and may participate in construction of negative feedback loop of sfks to inhibit lung cancer cells from growth,migration and invasion.