文章摘要
张晓洁,季晓辉,何 敏,陈赛英.妊娠不同时期胎盘单核-巨噬细胞来源的树突样细胞刺激t细胞作用的比较[J].南京医科大学学报,2009,29(9):1208~1214
妊娠不同时期胎盘单核-巨噬细胞来源的树突样细胞刺激t细胞作用的比较
Comparison of the characteristic of t-cell-stimulation between dendritic-like cells induced from monocyte-macrophages derived from placental tissue in different gestational ages
投稿时间:2009-04-08  
DOI:10.7655
中文关键词: 妊娠  胎盘  单核-巨噬细胞  树突状细胞  免疫
英文关键词: pregnancy  placenta  monocyte-macrophages  dendritic cells  immunity
基金项目:江苏省生殖医学重点实验室对外开放课题
作者单位
张晓洁 南京医科大学第一附属医院河西分院检验科,江苏 南京 210036 
季晓辉 南京医科大学微生物学与免疫学系,江苏 南京 210029 
何 敏 南京医科大学微生物学与免疫学系,江苏 南京 210029 
陈赛英 南京医科大学微生物学与免疫学系,江苏 南京 210029 
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中文摘要:
      目的:观察不同妊娠阶段胎盘源性树突样细胞刺激t细胞活化增殖特点的差异,为分析胎盘免疫细胞在妊娠耐受和分娩发动中的可能作用提供线索?方法:机械分离中?晚期胎盘中的单核-巨噬细胞,以内皮逆穿越系统诱导其分化为树突状细胞(dc)?将不同妊娠阶段胎盘单核-巨噬细胞来源的dc样细胞与同种异体t细胞共育,以cck-8法检测其激发同种异体t细胞增殖能力及其刺激同种异体t细胞所产生的胞内细胞因子,并用流式细胞仪分析dc样细胞表型?结果:足月胎盘来源的单核-巨噬细胞经过内皮单层正逆双向穿越诱导培养后,细胞出现树突状形态改变,并高表达与树突状细胞免疫激活有关的细胞表面标志物cd80?cd86? hla-dr?足月妊娠胎盘单核-巨噬细胞来源dc样细胞具有较强的刺激同种异体淋巴细胞增殖的能力,同种异体淋巴细胞活化时细胞内ifn-γ+亚型的表达十分明显,但几乎不出现il-10+ 亚型?但相比之下,中期妊娠胎盘单核-巨噬细胞来源的dc样细胞刺激同种异体t细胞增殖的能力却很弱(p < 0.05),被刺激的同种异体t细胞中cd8-(即cd4+)亚群ifn-γ+细胞显著减少(p < 0.05),而cd8-和cd8+亚群中il-10+ 细胞亚群却显著增多?对细胞的表型分析结果显示,中期妊娠胎盘单核-巨噬细胞来源的dc样细胞cd80?cd86及hla-dr的表达均显著低下(p < 0.05)?结论:不同妊娠时期胎盘单核-巨噬细胞分化形成具有免疫激活作用的树突状细胞的能力不同,提示其生物学特性有所不同,其可能与妊娠耐受和分娩发动有关?
英文摘要:
      objective:to compare the different features of t-cell-stimulation of dendritic-like cells (dc-like cells)induced from monocyte-macrophages derived from placentas in different gestational ages. Methods:monocyte-macrophages were separated mechanically from normal term placental tissues and second trimester placental tissues,then cultured in the transendothelial trafficking system and induced into dc-like cells. The dc-like cells were co-cultured with allogeneic t cells,in order to detect their abilities to stimulate allogeneic t cells into proliferation by cck-8 incorporation assay. Expressions of intracellular cytokines by stimulated t cells and the phenotype of dc-like cells were analyzed by facs. Results:after being cultured in the transendothelial trafficking system for 48 h,the cells which derived from the normal term placental monocyte-macrophages displayed characteristic morphology and expressed higher level surface antigen of cd80,cd86,hla-dr,and obviously stimulated the proliferation of allogeneic t cells,which could be led to a higher percentage of ifn-γ producing cells,but fewer of il-10 producing cells. However,dc-like cells,originated from second trimester placental tissues,expressed quite little levels of cd80,cd86 and hla-dr(p < 0.05)and stimulated very few allogeneic t cells to proliferation (p < 0.05). Furthermore,in the t cells stimulated by these dc-like cells ,there were small percentages of ifn-γ producing cells in cd8+ subset (p < 0.05)and large percentages of il-10 producing cells in both cd8- and cd8+ subsets. Conclusion:dc-like cells induced from monocyte-macrophages separated from different gestational ages placental tissues display functions in immunology with different biological characteristics.
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