文章摘要
朱利群,徐 珊,李 卓,徐昌芬.4’-甲醚-黄芩素逆转人绒毛膜癌耐药细胞株多药耐药性的相关机制研究[J].南京医科大学学报,2009,29(9):1215~1219
4’-甲醚-黄芩素逆转人绒毛膜癌耐药细胞株多药耐药性的相关机制研究
Reverse mechanism of 4’-methylether-scutellarein on multidrug resisatant human choriocarcinoma cell line
投稿时间:2009-03-25  
DOI:10.7655
中文关键词: 4’-甲醚-黄芩素  绒毛膜癌  多药耐药  耐药逆转  p-糖蛋白
英文关键词: 4’-methylether-scutellarein  choriocarcinoma  multidrug resistance  reversal of drug resistance  p-glycoprotein
基金项目:江苏省高校自然科学基金(06KJB360067)
作者单位
朱利群 南京医科大学江苏省生殖医学重点实验室,江苏 南京 210029 
徐 珊  
李 卓  
徐昌芬  
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中文摘要:
      目的:探讨4’-甲醚-黄芩素(4’-m-s)逆转人绒毛膜癌耐药细胞株jar/vp16多药耐药性的可能机制?方法:采用流式细胞术测定4’-m-s作用前后耐药细胞内罗丹明(rh123)潴留量的变化,以检测4’-m-s对p-糖蛋白(p-gp)功能的影响;用半定量逆转录聚合酶链反应(rt-pcr)检测多药耐药基因mdr1 mrna的表达,并进一步采用western blot及免疫组织化学法测定mdr1基因产物p-gp的表达?结果:流式细胞术结果表明,20 μg/ml 4’-m-s可明显增加耐药细胞内潴留的rh123浓度;rt-pcr及western blot结果显示,4’-m-s可抑制耐药细胞中mdr1 mrna和p-gp的表达;免疫组化实验显示,4’-m-s 处理后,耐药细胞中p-gp的表达下降,表现为细胞膜上阳性颗粒数量减少?着色变浅?结论:4’-m-s对人绒毛膜癌耐药细胞株多药耐药性的逆转,与抑制mdr1 mrna及其产物p-gp的表达?增加细胞内化疗药物的浓度有关?
英文摘要:
      objective:to investigate the reversal effect of 4’-methylether-scutellarein(4’-m-s) on multidrug resistance in human choriocarcinoma jar/vp16 cell line. Methods:quantitative accumulation and efflux of rhodamine 123 (rh123),a p-glycoprotein(p-gp)substrate,were determined using a flow cytometric assay (fcm) for measuring p-gp function. Mdr1 mrna levels were detected by semi-quantitative reverse transcriptase-polymerase chain reaction (rt-pcr). The expressions of p-gp were analyzed by western blot and immunohistochemistry(ihc) method. Results:the results of fcm suggested that 20 μg/ml 4’-m-s could significantly increase the intracellular accumulation of rh123 in jar/vp16 cells. Rt-pcr and western blot results revealed that the expression levels of mdr1 mrna and p-gp protein were low in 4’-m-s treated cells. The ihc study showed that mild immunostaining for p-gp was seen in jar/vp16 cells when treated with 4’-m-s. Conclusion:the reversal effect of 4’-m-s in jar/vp16 cell may be related to regulating expression of mdr1/p-gp and increaseing intracellular concentration of chemotherapeutic drugs.
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