Single nucleotide polymorphisms in STAT3 and its association with biological behavior of gastric cancer
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摘要:
目的:STAT(signal transducer and activators of transcription)是一种重要的细胞质转录因子,在恶性肿瘤的发生-增殖和凋亡中发挥着重要作用-本研究旨在探讨STAT3单核苷酸多态性(single nucleotide polymorphisms,SNP)与胃癌生物学行为的关系-方法:运用SYBR GREEN PCR熔解曲线法检测93例胃癌患者的STAT3 SNP-结果:随机抽取18例胃癌患者以基因测序检测STAT3 SNP,结果与SYBR GREEN PCR熔解曲线法完全相同-含有G等位基因的CG+GG基因型相对于CC基因型,脉管侵犯以及神经侵犯差异性显著(P < 0.001)-结论:SYBR GREEN PCR熔解曲线法可以作为检测STAT3 SNP方法之一-STAT3 SNP与胃癌的某些生物学行为相关,该位点为G等位基因提示肿瘤脉管侵犯-神经侵犯高风险-
Abstract:
Objective:STAT(signal transducer and activators of transcription) is one kind of cytoplasm transcription factor which plays an important role in promoting malignant tumor to genesis,proliferation and apoptosis. This research is to approach the association the single nucleotide polymorphisms(SNP)in STAT3 with biological behavior of gastric cancer. Methods:Using SYBR GREEN PCR with Tm-shift primers to detect genotyping of the SNP in STAT3 in 93 gastric cancer patients. Results:SNP in STAT3 of 18 gastric cancer patients,which confirmed by gene sequencing are same as the results by SYBR GREEN PCR with Tm-shift primers. Stratified analysis indicated that an elevated risk of vessel invasion and nerve invasion was observed in subjects who carried at least one G allete (GG or CG) compared with CC genotype (P < 0.001). Conclusion:SYBR GREEN PCR with Tm-shift primers can be one of the methods to detect STAT3 SNP. SNP in STAT3 was associated with clinicobiological behaviors of gastric cancer,such as vessel invasion and nerve invasion. The expression of STAT3 G allele indicates high risk of vessel invasion and nerver invasion.
