文章摘要
蔡旭兵,潘立群.黄芪微乳载入修饰后胶原对人脐静脉内皮细胞增殖的影响[J].南京医科大学学报,2009,29(12):1653~
黄芪微乳载入修饰后胶原对人脐静脉内皮细胞增殖的影响
Effects of Astragalus mongholicus microemulsion modified-collagen on proliferation of human umbilical vascular endothelial cells in vitro
投稿时间:2009-06-19  
DOI:10.7655
中文关键词: 黄芪微乳  胶原  人脐静脉内皮细胞  细胞增殖  血管内皮细胞生长因子
英文关键词: Astragalus mongholicus microemulsion  collagen  human umbilical vascular endothelial cell  cell proliferation  vascular endothelial cell growth factor
基金项目:国家自然科学基金资助(30572332)
作者单位
蔡旭兵 南京中医药大学,江苏 南京〓210029 
潘立群 江苏省中医院外科,江苏 南京〓210029 
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中文摘要:
      目的:研究黄芪微乳载入修饰后胶原对人脐静脉内皮细胞(HUVEC)增殖的影响,进一步探讨黄芪微乳对HUVEC增生的作用及其机制?方法:体外培养HUVEC细胞,用含1%FCS的RPMI-1640培养液同步24 h后,分为2组进行观察:空白微乳胶原组和黄芪微乳胶原组,并设定空白微乳和黄芪微乳的浓度分别为16.90?8.50?4.20?2.10?1.05 μg/ml等5个浓度,每组每个浓度设3个复孔?干预48 h后,采用MTT法和流式细胞术检测各组细胞的增殖情况;实时荧光定量RT-PCR检测细胞内血管内皮细胞生长因子(VEGF)mRNA表达?结果:干预48 h后,在浓度分别为16.9?8.5?4.2 μg/ml的黄芪微乳胶原组HUVEC的吸光度值?细胞分裂增殖指数和VEGF mRNA表达均明显高于相应浓度的空白微乳胶原组(P < 0.05,P < 0.01)?结论:黄芪微乳能促进HUVEC的增殖,并上调HUVEC的VEGF mRNA表达,推测黄芪微乳可能具有促进血管生成的作用?
英文摘要:
      Objective:To investigate the effect of Astragalus mongholicus microemulsion modified-collagen on proliferation of human umbilical vascular endothelial cells(HUVEC) in vitro. Methods:Cell line of HUVEC was cultured and all experiments were performed using the cells at the third passage. After synchronization of cells growth,HUVEC was divided into blank microemulsion modified-collagen group and Astragalus mongholicus microemulsion modified-collagen group. Each group was exposed to the concentrations of 16.90,8.50,4.20,2.10 and 1.05 μg/ml,respectively. The proliferation of HUVEC was measured by MTT and flow cytometry assay. The real-time quantitative PCR was used to detect the expression of vascular endothelial cell growth factor(VEGF) mRNA in HUVEC. Results:After co-culturing for 48 h,the proliferation and VEGF mRNA expression of HUVEC in Astragalus mongholicus microemulsion modified-collagen group at the concentrations of 16.9,8.5 and 4.2 μg/ml significantly increased compared with the groups on the same concentrations in blank microemulsion modified-collagen control(P < 0.05,P < 0.01). Conclusion:Astragalus mongholicus microemulsion not only promotes the proliferation of HUVEC,but also upregulates the expression of VEGF mRNA. This study demonstrateds that Astragalus mongholicus microemulsion may promote angei-anagenesis.
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