文章摘要
吴宏图,赵德育,周扬扬.儿童肺炎支原体肺炎MP-DNA检测和评价[J].南京医科大学学报,2010,(1):96~97101
儿童肺炎支原体肺炎MP-DNA检测和评价
Evaluation of MP-DNA quantitative detection in children with Mycoplasma Pneumoniae Pneumonia
投稿时间:2009-07-13  
DOI:10.7655
中文关键词: 肺炎支原体肺炎  MP-DNA  检测和评价
英文关键词: mycoplasma pneumoniae pneumonia  MP-DNA  detection and evaluation
基金项目:
作者单位
吴宏图 南京医科大学附属南京儿童医院呼吸科,江苏 南京〓210029 
赵德育 南京医科大学附属南京儿童医院呼吸科,江苏 南京〓210029 
周扬扬 淮安第二人民医院中心实验室,江苏 淮安〓223002 
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中文摘要:
      目的:探讨肺炎支原体DNA(MP-DNA),超敏C反应蛋白(SCRP)对诊断支原体肺炎的敏感性和特异性,为临床提供准确快速的诊断依据?方法:采用MP荧光定量PCR法检测患儿呼吸道分泌物标本中的MP-DNA水平,同时检测血清SCRP和MP-IgM的浓度,并与WBC计数进行比较?结果:MP-DNA和SCRP对诊断支原体肺炎的敏感性均高于WBC计数,3个指标的敏感性以SCRP最高;特异性和阳性预测值以MP-DNA最高;阴性预测值和约登指数,MP-DNA和SCRP两者相差无几?结论:MP-DNA检测对肺炎支原体的早期诊断有一定价值?
英文摘要:
      Objective:To explore diagnostic evaluation of sensitivity and specificity of MP-DNA and SCRP on children with MP pneumonia,and provide accurate and quick diagnostic proof to clinic. Methods:Children’s respiratory secretion samples were collected for fluorescent quantitative PCR for MP-DNA,and the serum’s concentrations of SCRP and MP-IgM were detected by immunobiochemistry methods,and the three indexes were compared with WBC’s count. Results:MP-DNA and sCRP were more sensitive in diagnosis of MPP compared with WBC count. Among the three indexes,the sCRP was the most sensitive,and MP-DNA had highest specificity and positive predictive value,and there were no differences among the indexes on negative predictive value and Youden’s index. Conclusion:MP-DNA is evaluable in early and accurate diagnosis on MP infection.
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