Objective:To explore diagnostic evaluation of sensitivity and specificity of MP-DNA and SCRP on children with MP pneumonia,and provide accurate and quick diagnostic proof to clinic. Methods:Children’s respiratory secretion samples were collected for fluorescent quantitative PCR for MP-DNA,and the serum’s concentrations of SCRP and MP-IgM were detected by immunobiochemistry methods,and the three indexes were compared with WBC’s count. Results:MP-DNA and sCRP were more sensitive in diagnosis of MPP compared with WBC count. Among the three indexes,the sCRP was the most sensitive,and MP-DNA had highest specificity and positive predictive value,and there were no differences among the indexes on negative predictive value and Youden’s index. Conclusion:MP-DNA is evaluable in early and accurate diagnosis on MP infection.