文章摘要
李志忠,孙国栋,吴波文,洪〓亮.BMSCs复合CS-CPC/BMP-2/bFGF支架材料的生物相容性研究[J].南京医科大学学报,2010,(2):166~169190
BMSCs复合CS-CPC/BMP-2/bFGF支架材料的生物相容性研究
Biocompatibility between CS-CPC/BMP-2/bFGF composite material scaffolds and bone marrow mesenchymal stem cells after induction
  
DOI:10.7655
中文关键词: 壳聚糖-磷酸钙  细胞生长因子  骨髓间充质干细胞  生物相容性
英文关键词: chitosan-calcium-phosphate cement  cell growth factor  BMSCs  biocompatibility
基金项目:
作者单位
李志忠 暨南大学附属第一医院骨科,广东 广州〓510632 
孙国栋  
吴波文  
洪〓亮  
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中文摘要:
      目的:研究CS-CPC/BMP-2/bFGF支架材料的生物相容性,为骨组织工程提供一种新型复合支架材料?方法:制备CS-CPC/BMP-2/bFGF支架材料?将大鼠骨髓间充质干细胞(BMSCs)与CS-CPC/BMP-2/bFGF支架材料联合培养,体外成骨诱导培养2周,扫描电镜观察细胞黏附情况,MTT法分析细胞增殖情况以评价其组织相容性?结果:扫描电镜示细胞在新型支架上吸附?生长良好?MTT示BMSCs在材料上和单纯诱导的细胞增殖能力基本相同?结论:CS-CPC/BMP-2/bFGF支架材料是一种具有良好生物相容性的支架材料,有望在骨组织工程中得到广泛应用?
英文摘要:
      Objective:To study the compatibility between CS-CPC/BMP-2/bFGF composite material scaffolds and bone marrow mesenchymal stem cells after induction and to explore a new scaffold for bone tissue engineering. Methods:The CS-CPC/BMP-2/bFGF composite material scaffolds were produced and co-cultured with bone marrow mesenchymal stem cells,then these composite material scaffolds were cultured in osteogenic medium for two weeks,and the growth and adhesion of cells on the scaffold were observed with scanning electron microscope and the cell proliferation was detected by MTT assay so as to evaluate the tissue response. Results:The cells grew well on the surface of the CS-CPC/BMP-2/bFGF composite material scaffolds. The MTT test assay showed that the CS-CPC/BMP-2/bFGF composite material scaffolds couldn’t inhibit the proliferation of BMSCs. Conclusion:The CS-CPC/BMP-2/bFGF composite material scaffolds has good biocompatibility which showed the potential of novel scaffold for bone tissue engineering.
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