文章摘要
王〓敏,姚〓欣,黄〓茂.雷公藤甲素对外周血单个核细胞白细胞介素17A分泌的影响[J].南京医科大学学报,2010,(3):316~318393
雷公藤甲素对外周血单个核细胞白细胞介素17A分泌的影响
The effect of triptolide on the release of interleukin 17A in human peripheral blood mononuclear cells
  
DOI:10.7655
中文关键词: 支气管哮喘  白细胞介素17A  外周血单个核细胞
英文关键词: asthma  IL-17A  PBMC
基金项目:国家自然科学基金资助项目(30700342)
作者单位
王〓敏 南京医科大学第一附属医院呼吸科,江苏 南京〓210029 
姚〓欣  
黄〓茂  
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中文摘要:
      目的:探讨外周血单个核细胞(PBMCs)经CD3?CD28双信号通路活化后白细胞介素17A(IL-17A)的分泌以及免疫调节剂雷公藤甲素对该细胞因子分泌的影响?方法:抗CD3单克隆抗体(浓度10 μg/ml)和抗CD28单克隆抗体(浓度5 μg/ml)共刺激PBMCs,模拟T细胞在体内受双信号免疫活化的过程,应用酶联免疫吸附方法(ELISA)检测细胞培养液上清IL-17A水平?结果:外周血单个核细胞经CD3/CD28双信号通路刺激后,其分泌的IL-17A在6 h内即出现明显增加,并在24 h内呈继续增高的趋势;100 nmol/L和500 nmol/L浓度的雷公藤甲素均能够显著抑制该细胞分泌IL-17A?结论:PBMCs经双信号免疫活化后,其分泌的IL-17A明显增加,可能是哮喘气道变应性炎症的形成和发展机制之一;雷公藤甲素能够通过抑制PBMCs对IL-17A分泌,从而有可能减轻哮喘气道炎症?
英文摘要:
      Objective:To investigate the effect of anti-CD3 plus anti-CD28 antibody co-stimulation on the release of interleukin-17 A (IL-17A) in human peripheral blood mononuclear cells (PBMCs). And the effect of triptolide on IL-17A release was also studied. Methods:PBMCs were stimulated with anti-CD3 monoclonal antibody (10 μg/ml) and anti-CD28 monoclonal antibody (10 μg/ml). ELISA was applied to measure the concentration of IL-17A in the supernatant of the cell culture. Results:IL-17A was released in time-dependant manner by PBMCs due to anti-CD3 plus anti-CD28 co-stimulation,however the release could be inhibited by triptolide at the concentration of either 100 nmol/L or 500 nmol/L. Conclusion:IL-17A produced by PBMCs might play an important role in the mechanism of asthma. Triptolide might inhibit the Th2 type allergic inflammation in asthma through reducing the release of the IL-17A.
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