文章摘要
欧红芹,马向华,沈〓捷,沈海军.白细胞介素6对人单核/巨噬细胞系细胞表面Toll样受体4表达的影响[J].南京医科大学学报,2010,(3):319~323
白细胞介素6对人单核/巨噬细胞系细胞表面Toll样受体4表达的影响
Effects of human interleukin-6(IL-6) on the expression of Toll like receptor-4(TLR4) of THP-1
投稿时间:2009-10-12  
DOI:10.7655
中文关键词: IL-6  THP-1  TLR4
英文关键词: IL-6  THP-1  TLR4
基金项目:江苏省卫生厅创新基金(XK12200903)
作者单位
欧红芹 南京医科大学第一附属医院内分泌科,江苏 南京〓210029 
马向华  
沈〓捷  
沈海军  
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中文摘要:
      目的:观察白细胞介素6(IL-6)对人单核/巨噬细胞系(THP-1)细胞表面Toll样受体4(TLR4)表达的影响?方法:流式细胞仪检测不同浓度IL-6(0 ng/L?10 ng/L?100 ng/L?1 μg/L?10 μg/L?100 μg/L)干预THP-1细胞24 h,以及10 μg/L的IL-6干预THP-1细胞不同的时间(0?3?6?12?24?48 h)后细胞表面TLR4的表达水平?结果:各浓度IL-6均能不同程度的促进细胞表面TLR4的表达(P < 0.05),以10 μg/L 组干预最为显著(P < 0.01);10 μg/L IL-6干预不同的时间后均可促进其表达,以干预24 h后的差异最显著(P < 0.01)?结论:IL-6可以促进人THP-1细胞表面TLR4表达,且具有一定的浓度和时间依赖性?
英文摘要:
      Objective:To explore the effects of IL-6 on the expression of Toll like receptor-4(TLR4) of THP-1. Methods:Flow Cytometry(FCM) was used to detect the expression of TLR4 of THP-1 cells,which was stimulated with different concertrations of IL-6(0 ng/L,10 ng/L,100 ng/L,1 μg/L,10 μg/L and 100 μg/L),and on different time after treated with 10 μg/L IL-6 (0 h,3 h,6 h,12 h,24 h,48 h). Results:Different concertrations of IL-6 can significantly promote the expression of TLR4 in THP-1 cells,especially under the concentration of 10 μg/L IL-6 group. Still this effect can be observed in 10 μg/L IL-6 group under treated for 3 h, 6 h,12 h,24 h and 48 h,especially at 24 h. Conclusion:The expression of of TLR4 on THP-1 cells can be promoted by IL-6 in a dose-and time-dependence.
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