文章摘要
杨学文,张军峰,许冬青,董〓伟,詹〓瑧.EGF和TGF-α对舌鳞癌细胞周期调控的信号通路研究[J].南京医科大学学报,2010,(4):445~449554
EGF和TGF-α对舌鳞癌细胞周期调控的信号通路研究
Study on the signal pathway of EGF and TGF-α modulating the distribution of cell cycle in tongue squamous cell
投稿时间:2009-12-21  
DOI:10.7655
中文关键词: EGF  TGF-α  舌鳞癌细胞  细胞周期  信号通路
英文关键词: EGF  TGF-α  tongue squamous cell  cell cycle  signal pathway
基金项目:国家自然科学基金(30472123,30973715);江苏省自然科学基金(BK2008461);南京中医药大学青年自然科学基金(07XZR01)
作者单位
杨学文 南京中医药大学中西医结合学科,江苏 南京〓210046 
张军峰  
许冬青  
董〓伟  
詹〓瑧  
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中文摘要:
      目的:研究EGF和TGF-α调控舌鳞癌细胞周期分布的信号通路?方法:不同浓度EGF和TGF-α作用体外培养的舌鳞癌细胞株TCA-8113,流式细胞仪检测细胞周期分布,细胞免疫组化法研究p38-MAP kinase?c-myc?c-fos和NF-κB在舌鳞癌细胞中的表达水平?结果:EGF和TGF-α效应类似,与对照组相比,G1/G0期细胞比例下降,S期细胞比例增加,p38-MAP kinase?c-myc和c-fos表达水平下调,NF-κB p65表达水平上调,但量效关系不同?EGF和TGF-α分别上调和下调NF-κB p50表达?结论:EGF和TGF-α对舌鳞癌细胞周期分布的影响差异与NF-κB相关,NF-κB可能与舌鳞癌发生关系密切?
英文摘要:
      Objective:To investigate the signal pathway of EGF and TGF-α modulating the distribution of cell cycle in tongue squamous cell. Methods:Flow cytometric analysis and immunohistochemistry were employed to investigate the cell cycle distribution,and expressed levels of p38-MAP kinase,c-myc,c-fos and NF-κB respectively. Results:EGF and TGF-α showed similar effect on tongue squamous cell,but the dose-effect relationships were different. In comparison with the control,the proportion of cells in G1/G0 declined and that in S phase ascended. The levels of p38-MAP kinase,c-myc and c-fos were down-regulated and NF-κB p65 up-regulated. EGF and TGF-α up- and down-regulated the expressed levels of NF-κB p50 respectively. Conclusion:The distinct expression of NF-κB may be one of the reasons that results in the difference of EGF and TGF-α on the cell cycle distribution. NF-κB is closely related to the occurrence of tongue squamous carcinoma.
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