文章摘要
龙卫国,郭 泽,曹伯良,张爱霞.探讨不同实验条件对U87细胞体外增殖的影响[J].南京医科大学学报,2010,(5):632~635724
探讨不同实验条件对U87细胞体外增殖的影响
Effects of cell culture conditions on proliferation assay of U87 cells
  
DOI:10.7655
中文关键词: RNA干扰  细胞增殖  培养条件
英文关键词: RNA interference  cell proliferation  culture conditions
基金项目:南京医科大学科技发展重点基金项目资助(07NMUZ005);横向合作项目(Van Andel Institute)(ky1011711084000092)
作者单位
龙卫国 南京医科大学病理学系,卫生部抗体技术重点实验室,江苏 南京 210029 
郭 泽  
曹伯良  
张爱霞  
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中文摘要:
      目的:探讨RNA干扰肝癌衍生生长因子(HDGF)后,U87细胞增殖抑制的最佳实验条件?方法:用LipofectamineTM 2000将HDGF siRNA转染U87细胞后,将细胞接种于96孔板中,分别在无血清?含10%FBS和无血清Matrigel胶预处理细胞培养板条件下,采用MTS法检测细胞增殖能力?结果:HDGF表达水平下调后,U87细胞增殖能力受到抑制?在无血清?含10%FBS和无血清Matrigel胶预处理细胞培养板条件下,细胞增殖抑制率分别是18%?9%和28%?结论:在无血清Matrigel胶预先处理的培养条件下,能最大程度上反映出HDGF siRNA对U87细胞增殖能力的抑制?
英文摘要:
      Objective:To optimize the culture conditions of glioma U87 cells for proliferation inhibiting assay. Methods:After down-regulation of HDGF expression by RNA interference in U87 cell line,cells were inoculated in 96-well plate. MTS cell proliferation assay was performed under three different conditions including serum free culture condition,containing 10% FBS culture condition and plate precoated with Matrigel. Results:U87 cell proliferation was inhibited after down-regulation of HDGF level. Cell proliferation inhibiting rates in serum free group,10% FBS group and Matrigel precoated group were 18%,9%,28%,respectively. Conclusion:Pre-coating the plate with Matrigel may be optimal for cell proliferation inhibiting detection after RNA interference.
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