文章摘要
李秀珍,谭若芸,鲁 翔.解耦联蛋白2对糖尿病小鼠心肌细胞凋亡相关蛋白表达的影响[J].南京医科大学学报,2010,(5):646~648683
解耦联蛋白2对糖尿病小鼠心肌细胞凋亡相关蛋白表达的影响
Effects of uncoupling protein 2 on the expression of myocardial apoptotic protein of diabetic mellitus in mice
  
DOI:10.7655
中文关键词: 解耦联蛋白2  糖尿病  心肌  凋亡
英文关键词: uncoupling protein 2  diabetic mellitus  myocardium  apoptosis
基金项目:江苏省科技厅社会发展基金(BS2006007)
作者单位
李秀珍 南京医科大学第一附属医院心内科,江苏 南京 210029 
谭若芸 南京医科大学第二附属医院肾内科,江苏 南京 210011 
鲁 翔 南京医科大学第一附属医院心内科,江苏 南京 210029 
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中文摘要:
      目的:研究解耦联蛋白2(uncoupling protein 2,UCP2)与糖尿病(diabetes mellitus,DM)小鼠心肌细胞凋亡的关系?方法:用雄性UCP2敲除小鼠(UCP2-/-)及C57BL/6小鼠建立糖尿病模型,各组分别饲养7天及28天?采用Hochest荧光染色观察糖尿病小鼠心肌细胞形态学的变化,Western blot方法检测凋亡蛋白caspase3的表达?结果:① DM28天组 Hochest染色细胞出现核固缩状和颗粒状荧光等典型的凋亡学特征?DM28天组较DM7天组染色质明显浓缩,荧光强度明显增加?UCP2-/-+DM7天组较DM7天组染色质浓缩减轻,颗粒状荧光减少;UCP2-/-+DM28天组较DM28天组染色质浓缩明显减轻,颗粒状荧光明显减少?② Western blot结果显示:UCP-/-+DM 7天组cleaved-caspase 3蛋白表达较DM 7天组稍下降,无统计学意义(P > 0.05);UCP2-/-+DM28天组cleaved-caspase3蛋白表达较DM28天组明显减少,(P < 0.05)?结论:UCP2与糖尿病心肌细胞凋亡存在密切关系?
英文摘要:
      Objective:To observe the relationship between myocardial apoptosis of diabetic mellitus and uncoupling protein 2 (UCP2). Methods:Diabetic animal models were induced by streptozotocinon on UCP2 knock-out mice(UCP2-/-) and C57BL/6 mice,rearing for 7 and 28 days each group. The expressions of myocardial apoptosis relative protein,caspase 3,were investigated using western blot. Hochest fluorescence staining detected morphological changes of myocardial cells in left venticle of diabetic mice. Results:Hochest staining results showed cells nuclear condensation,granular fluorescence and other typical features of apoptosis in DM-28-day group. Myocardium of diabetic mice exhibited significant concentration of chromatin at 7 days and aggravated at 28 days,with high fluorescence intensity. However,slight concentration of chromatin was detected on UCP2-/-+DM mice comparison of DM mice at 28 days. Image analysis showed that the expression of cleaved-caspase 3 protein levels decreased slightly in UCP-/-+DM-7-day group comparing with DM-7-day group(P > 0.05). The expression of cleaved-caspase 3 protein levels decreased significantly(P < 0.05)in UCP-/-+DM-28-day group comparing with DM-28-day group. Conclusion:UCP2 may be one of the most important factors which contribute to myocardial apoptosis of diabetic mellitus.
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