文章摘要
杨 璟,张国新,郝 波,施瑞华,徐顺福.胃癌癌旁组织中NF-κB?IL-8与CagA的表达[J].南京医科大学学报,2010,(5):667~672
胃癌癌旁组织中NF-κB?IL-8与CagA的表达
The expression of NF-κB,IL-8 and CagA in the peri-cancer tissues of gastric cancer
  
DOI:10.7655
中文关键词: 细胞毒素相关蛋白  核因子κB  白细胞介素8  胃癌  慢性胃炎
英文关键词: cytotoxin-assocated protein A  nuclear factor kappa B  interleukin-8  gastric cancer  chronic gastritis
基金项目:2007江苏省“科教兴卫工程”医学重点学科资助
作者单位
杨 璟 南京医科大学第一附属医院消化科,江苏 南京 210029 
张国新  
郝 波  
施瑞华  
徐顺福  
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中文摘要:
      目的:观察胃癌癌旁组织中核因子κB(NF-κB)?白细胞介素8(IL-8)的表达与幽门螺杆菌的毒力因子细胞毒素相关蛋白(CagA)的关系,探讨CagA在胃癌发生?发展过程中的作用?方法:胃癌手术切除标本58例(均经病理证实为胃腺癌),胃镜下慢性胃炎标本54例(均经病理证实无萎缩及不典型增生)?应用免疫组化(SP)和Western blot法分别检测胃癌癌旁组织及慢性胃炎组织中CagA?NF-κB?IL-8的表达,并分析胃癌癌旁组织中NF-κB?IL-8的表达与CagA的关系?结果:慢性胃炎?胃癌癌旁组织中CagA阳性率分别为53.7%(29/54)?62.1%(36/58),两组间无显著差异(P > 0.05);癌旁组织中NF-κB及IL-8阳性率高于慢性胃炎组织(P < 0.05)?CagA阳性慢性胃炎组织中NF-κB及IL-8的表达与CagA阴性组织无明显差异(P > 0.05);CagA阳性癌旁组织中NF-κB及IL-8的表达高于CagA阴性癌旁组织(P < 0.05)?计数胃癌癌旁组织中NF-κB?IL-8及CagA平均阳性细胞比率,经相关性分析提示NF-κB及IL-8的表达均与CagA的表达呈正相关(P < 0.05)?结论:癌旁组织反映了癌前状态或病变,癌旁组织中NF-κB及IL-8高表达与CagA有关?由此推断CagA可能通过诱导NF-κB及IL-8的表达,在胃癌的发生与发展过程中起重要作用?
英文摘要:
      Objective:To determine the relationship between expression of nuclear factor kappa B(NF-κB),interleukin-8(IL-8) and cytotoxin-associated protein A(CagA),and evaluate the clinical implication of CagA in tumorigenesis and progression of gastric carcinoma. Methods:Tissue specimens from 54 cases of chronic gastritis and 58 cases of gastric cancer were evalutaed for the expression of CagA,NF-κB and IL-8 by immunohistochemistry and western blot. The relationship between NF-κB,IL-8 and CagA were detected by correlation analysis. Results:There was no difference in positive rate of CagA protein between chronic gastritis and gastric cancer groups(53.7% vs 62.1%,P > 0.05). The positive rate of NF-κB and IL-8 were significant higher in peri-cancer tissues of gastric cancer than that in chronic gastritis(P < 0.05). In the peri-cancer tissues of gastric cancer,the expression of NF-κB and IL-8 were significant higher in CagA positive group than that of CagA negative group(P < 0.05). And there was a positive correlation between the expression of NF-κB,IL-8 and CagA. Conclusion:CagA protein might be important in tumorigenesis and progression of gastric carcinoma by up-regulating the expression of NF-κB and IL-8.
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