文章摘要
黄汉鹏,庄〓燕,柏〓惠,杨〓青,陈〓琪.ERK信号通路参与内质网应激时由A类清道夫受体介导的细胞凋亡[J].南京医科大学学报,2010,(6):731~735
ERK信号通路参与内质网应激时由A类清道夫受体介导的细胞凋亡
Contribution of ERK signaling to class A scavenger receptor-induced apoptosis in the endoreticulum stessed macrophages
投稿时间:2010-05-03  
DOI:10.7655
中文关键词: fucoidan  ERK  内质网应激  凋亡
英文关键词: fucoidan  ERK  ER stress  apoptosis
基金项目:国家自然科学基金重点项目(30730044)
作者单位
黄汉鹏 南京医科大学动脉粥样硬化研究中心,江苏 南京〓210029 
庄〓燕  
柏〓惠  
杨〓青  
陈〓琪  
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中文摘要:
      目的:探讨ERK信号通路在内质网应激时由A类清道夫受体(SR-A)介导的细胞凋亡中的作用?方法:ERK磷酸化和葡萄糖相关蛋白78(GRP78)的表达用Western blot方法检测,细胞活性用四甲基噻唑蓝(MTT)实验检测,同时应用Annexin V FITC/PI 双染流式细胞术检测细胞凋亡率?结果:Western blot结果显示,fucoidan可以持续激活ERK,并且在1~4 h达到最强,这种作用在SR-A基因敲除小鼠的腹腔巨噬细胞中明显减弱?用PD98059阻断ERK信号途径后,内质网应激时fucoidan诱导RAW264.7细胞凋亡率明显降低?结论:在发生内质网应激时,fucoidan与SR-A结合后激活ERK信号通路,可能是促进巨噬细胞凋亡的机制之一?
英文摘要:
      Objective:To investigate the effects of ERK signaling passway on the class A scavenger receptor(SR-A)-mediated apoptosis in the endoreticulum stessed macrophages. Methods:The expression of phosphorylation of ERK1/2 and GRP78 was measured by Western blot;MTT analysis was used to detect the surviving fraction of RAW264.7 cells treated with different concentrations of PD98059;and apoptosis rates of RAW264.7 cells were observed by flow cytometry after staining of Annexin V FITC/ PI. Results:Fucoidan continuously activated p-ERK,and the expression of p-ERK kept on peak level through 1 to 4 hours,but was low in the SR-A gene knockout macrophages. Under ER stress,fucoidan-induced apoptosis rates of RAW264.7 cells were significantly decreased by PD98059. Conclusion:These results demonstrated that under ER stress,fucoidan could activate the ERK pathway through SR-A;and activation of p-ERK could contribute to the apoptosis of macrophages.
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