文章摘要
胡阳英,沈美萍.钠/碘转运体在分化型甲状腺癌及其淋巴转移灶中的表达和分布[J].南京医科大学学报,2010,(8):1082~1085
钠/碘转运体在分化型甲状腺癌及其淋巴转移灶中的表达和分布
The expression and location of sodium/iodide symporter(NIS) in differentiated thyroid carcinoma and corresponding lymph nodes metastasis
投稿时间:2009-12-31  
DOI:10.7655
中文关键词: 钠/碘转运体  分化型甲状腺癌  淋巴转移灶
英文关键词: sodium/iodide symporter  differentiated thyroid carcinoma  lymph nodes metastasis
基金项目:江苏省科技厅国际合作计划资助(BZ2009053)
作者单位
胡阳英 南京医科大学第一附属医院普外科,江苏 南京〓210029 
沈美萍 南京医科大学第一附属医院普外科,江苏 南京〓210029 
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中文摘要:
      目的:研究钠/碘转运体(sodium/iodide symporter,NIS)在分化型甲状腺癌及其淋巴转移灶中的表达情况,探讨NIS与分化型甲状腺癌的关系及放射碘治疗分化型甲状腺癌的意义?方法:用实时荧光定量聚合酶链反应(real time polymerase chain reaction,RT-PCR)法及组织芯片免疫组化法检测分化型甲状腺癌原发灶,癌旁组织及其淋巴转移灶中NIS表达情况?结果:RT-PCR示:癌旁组织和分化型甲状腺癌原发灶NIS的mRNA表达量无显著性差异,转移淋巴结组NIS mRNA表达较其他组低?免疫组化示:NIS在分化型甲状腺癌原发灶?癌旁组织及淋巴转移灶中NIS阳性表达率无统计学差异,但是NIS在分化型甲状腺癌原发灶及淋巴转移灶胞膜中的表达比在癌旁组织胞膜中的表达低?结论:在甲状腺分化型癌中,NIS表达量正常,但NIS蛋白在细胞中的定位发生了改变,主要表达在细胞浆内,并非表达在功能靶位细胞膜上,此结果对放射碘治疗甲状腺分化型癌有指导意义?
英文摘要:
      Objective:To study the gene expression of sodium/iodide symporter(NIS) in both differentiated thyroid carcinoma(DTC)and correlated lymph nodes metastasis,and explore the relationship between NIS expression and DTC,and between NIS expression and the significance of radioactive iodine treatment for DTC. Methods:The expression of NIS in tissue specimens was evaluated by RT-PCR technique and immunohistochemistry. Results:The RT-PCR result showed that the expression of NIS mRNA didn’t have significant difference between tumor-adjacent tissues and DTC tissues. The NIS mRNA expression in metastatic lymph nodes were lower compared with tumor and tumor-adjacent tissues. The immunohistochemistry result showed that NIS positive ratio in DTC and correlated lymph nodes tissue didn’t have significant difference compared with that in normal thyroid,respectively. Conclusion:The NIS could be normally expressed,but NIS protein was mostly expressed in the cytoplasm rather than in the functional membrane,which has guiding significance for the radioiodine treatment of DTC.
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