文章摘要
陈胜平,吴浩荣,吴锦昌.绿色荧光蛋白标记的肝细胞移植治疗放射性肝损伤[J].南京医科大学学报,2010,(8):1100~1104
绿色荧光蛋白标记的肝细胞移植治疗放射性肝损伤
Treatment of radiation-induced liver damage in rats by transplantation of hepatocyte labled with green fluorescent protein
投稿时间:2010-01-13  
DOI:10.7655
中文关键词: 肝细胞  移植  放射性肝病  绿色荧光蛋白
英文关键词: hepatocyte  transplantation  radiation-induced liver disease(RILD)  green fluorescent protein(GFP)
基金项目:苏州市科技发展计划项目(SZD0832)
作者单位
陈胜平 昆山市第三人民医院普外科,江苏 昆山〓215300 
吴浩荣 苏州大学附属第二医院普外科,江苏 苏州〓215004 
吴锦昌 南京医科大学附属苏州市立医院放疗科,江苏 苏州〓214000 
摘要点击次数: 1281
全文下载次数: 167
中文摘要:
      目的:研究移植肝细胞在放射性肝损伤动物模型体内的代谢支持作用,评价绿色荧光蛋白标记的肝细胞移植对放射性肝病(radiation-induced liver disease,RILD)的治疗效果?方法:以60Co作为放射源照射实验大鼠肝区,总剂量40 Gy,一次性照射,剂量率为100 cG/min,制成急性放射性肝损伤动物模型?4天后将所有照射动物施行部分肝叶切除术(约占2/3)?将上述动物随机分为两组,实验组经脾脏实质行Ad-EGFP感染的肝细胞移植,移植细胞数为5.0×106个,对照组经脾实质注射等量生理盐水?术后7天两组分别采血测血清清蛋白水平?术后42天再次检测清蛋白,并作常规病理切片及透射电镜检查,在荧光显微镜下观察发绿光的肝细胞的分布,比较两组在组织结构上的差异?动态观察两组生存率的变化?结果:肝细胞移植术后7天,实验组血清清蛋白水平明显上升,和对照组相比有显著差异?移植后42天,实验组血清清蛋白水平进一步升高,与对照组?5 周前的实验组水平均有显著差异?移植后42天两组动物死亡率的差异有统计学意义?组织学检查示实验组放射性肝损伤轻于对照组?结论:同种肝细胞经脾脏移植后,在放射性肝损伤动物模型体内有良好的代谢支持作用,并可提高其短期生存率?
英文摘要:
      Objective:To study the metabolic support function of transplanted hepatocytes labled by green fluorescent protein in the animal model of radiation-induced liver damage(RILD) in vivo,and evaluate the therapeutic effects of transplanted hepatocytes in RILD. Methods:The models were constructed by exposing rats liver to 60Co irradiation,the dose being 40 Gy,and the dose ratio 100 cG/min. Four days later,the rats underwent partial hepatectomy,and were divided into two groups randomly. In the experimental group,5.0×106 hepatocytes transfected by Ad-EGFP were injected into the spleens while the control underwent physiologic saline injection in the same places. Blood samples were obtained at an interval of 5 weeks to measure the serum albumin level of the two groups,and histopathological changes were evaluated by light and electron microscopic examination. The survival rates of the two group were observed dynamically. Results:One week after hepatocytes transplantation(HT),serum albumin level of the experimental group ascended obviously,having significant differences with that of the control. It still ascended progressively 5 weeks later,showing significant differences with that of the control,as well as itself 5 weeks earlier. The differences of mortality rates between the two groups in 1 week and in 5 weeks after HT both show statistical significance. Histological examination suggested that radiative damage were ameliorated in the experimental group compared with that in the control. Conclusion:After transplanted into the spleens of the RILD model,congeneic rat hepatocytes can provide fairly good metabolic support and raise the short-term survival rate in the rat model.
查看全文   查看/发表评论  下载PDF阅读器
关闭