文章摘要
姜锡男,陈方敏,石家齐,严〓波,谷〓江,张〓亚,李登宝,沈〓俊,梁〓骏,任德帅.Wistar大鼠急性肾脏缺血再灌注损伤动物模型的建立及意义[J].南京医科大学学报,2010,(8):1108~1111
Wistar大鼠急性肾脏缺血再灌注损伤动物模型的建立及意义
Model construction of renal acute ischemia-reperfusion injury in Wistar rats
投稿时间:2010-01-22  
DOI:10.7655
中文关键词: 急性肾脏缺血再灌注损伤  动物模型
英文关键词: renal acute ischemia-reperfusion injury  animal model
基金项目:贵州省优秀科技教育人才省长基金专项基金项目(黔省专合字2006-59),贵阳医学院附属医院博士启动基金资助
作者单位
姜锡男 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
陈方敏 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
石家齐 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
严〓波 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
谷〓江 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
张〓亚 贵州省肿瘤医院妇瘤外科,贵州 贵阳〓550004 
李登宝 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
沈〓俊 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
梁〓骏 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
任德帅 贵阳医学院附属医院泌尿外科,贵州 贵阳〓550004 
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中文摘要:
      目的:建立一种简单?安全?高成功率的大鼠急性肾脏缺血再灌注损伤动物模型?方法:应用微型动脉夹夹闭Wistar大鼠双侧肾动脉50 min后松开,于伤后第3?5?7天取肾,经一系列处理后进行HE染色及免疫组化染色,以观察在肾脏近曲小管上皮细胞中是否出现肾损伤分子-1(kidney injury molecule-1,Kim-1)抗原及该细胞形态学改变?结果:该法造模成功率达93.3%,实验组肾脏近曲小管上皮细胞Kim-1抗原表达率上升,同时该细胞有明显细胞形态学改变(P > 0.05)?结论: 应用微型动脉夹夹闭Wistar大鼠双侧肾动脉可制备急性肾脏缺血再灌注损伤模型,且成功率高?方法简单?
英文摘要:
      Objective:To construct a simple,safe,high successful rate model of renal acute ischemia-reperfusion injury in Wistar rats. Methods:Renal artery of Wistar rats was bilaterally clamped with a micro-artery clamp for 50 minutes to make the animal model of renal acute ischemia-reperfusion injury. Then these kidneys were removed at the 3rd,5th,7th day after injury. Immunohistochemistry staining was used to show the distribution and expression of Kim-1 antigen,and the changes of cytomorphology were observed by HE staining. Results:The successful rate of model construction reached to 85% in the experiment. The expression of Kim-1 antigen increaced. Meanwhile,changes of cytomorphology took place. Conclusion:Stable model of renal acute ischemia-reperfusion injury of Wistar rats can be constructed by applying micro-artery clamp to incarcerate bilateral renal arteries,and its successful rate is very high.
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