文章摘要
李世芬,王心如,王玉翠,胡〓奇,赵〓岩.SD大鼠糖尿病肾病模型构建的比较[J].南京医科大学学报,2010,(8):1123~1128
SD大鼠糖尿病肾病模型构建的比较
Comparison different model construction methods of diabetic nephropathy SD rats
投稿时间:2010-03-01  
DOI:10.7655
中文关键词: 大鼠  链脲佐菌素  单侧肾脏切除  单侧肾脏结扎  糖尿病肾病
英文关键词: rat  STZ  uilateral nephritic excision  unilateral nephritic ligation  diabetic nephropathy
基金项目:
作者单位
李世芬 南京医科大学公共卫生学院,江苏 南京〓210029 
王心如 南京医科大学公共卫生学院,江苏 南京〓210029 
王玉翠 南京医科大学公共卫生学院,江苏 南京〓210029 
胡〓奇 南京医科大学公共卫生学院,江苏 南京〓210029 
赵〓岩 南京医科大学公共卫生学院,江苏 南京〓210029 
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中文摘要:
      目的:探讨不同方法建立糖尿病肾病(diabetic nephropathy,DN)模型的差异,以确定建立糖尿病肾病大鼠模型的最佳方案?方法:清洁级健康雌性SD大鼠30只,随机分为3组,每组10只?A组大鼠单侧肾脏切除后腹腔注射链脲佐菌素(streptozotocin,STZ)55 mg/kg,B组大鼠单侧肾脏结扎后腹腔注射STZ 55 mg/kg,C组为假手术对照组,注射等量的缓冲液?大鼠造模后从第1周开始每2周称体重,取血测血糖(Glu)?血肌酐(Scr)?总胆固醇(Chol)和甘油三酯(TG),收集尿测24 h尿蛋白(24Upro)?尿肌酐(Ucr),并计算内生肌酐清除率(Ccr)?11周后,处死大鼠,取肾脏称重并进行肾脏病理?结果:A?B 2组的成模周期相当,B组的存活率高于A组?结论:单侧肾脏结扎后再腹腔注射55 mg/kg STZ可以用来作为建立糖尿病肾病大鼠模型的理想方案?
英文摘要:
      Objective:To investigate the different model construction methods in diabetic nephropathy(DN) SD rats induced by streptozotocin(STZ) and establish the optimal plan. Methods:Thirty SD rats were randomly divided into 3 groups with 10 rats in each group. A group received intraperitoneal STZ after unilateral nephritic excision;B group received intraperitoneal STZ after unilateral nephritic ligation;C group(control group) received buffer solution only. The changes of body weight(BW),glucose(Glu),serum creatinine (Scr),cholesterol(Chol),triglyceride (TG)and 24-urine protein(24Upro),urine crea(Ucr)were detected every 2 weeks and creatinine clearance rate(Ccr) was calculated. After 11 weeks,the rats were sacrificed,and the kidneys were weighed and taken for pathological examination. Results:There was no significant difference in the model construction periods between the groups A and B. The survival rate of the group B is higher than that of the group A. Conclusion:STZ(55 mg/kg) intraperitoneal injection after unilateral nephritic ligation is the optimal method for inducing rat diabetic nephropathy.
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