文章摘要
朱晓波,闫智宏,徐志伟.维生素A结合胰岛素干预对STZ糖尿病大鼠血糖的影响[J].南京医科大学学报,2010,(8):1129~1133
维生素A结合胰岛素干预对STZ糖尿病大鼠血糖的影响
Effect of combination therapy with vitamin A and insulin on the blood glucose in STZ diabetic rats
投稿时间:2010-01-07  
DOI:10.7655
中文关键词: Vit A  胰岛素  糖尿病  大鼠  血糖
英文关键词: Vitamin A  insulin  diabetes  rat  blood glucose
基金项目:河北省科学技术研究与发展计划项目(07276101D-4)
作者单位
朱晓波 河北北方学院检验系生化教研室,河北 张家口〓075000 
闫智宏 河北北方学院检验系生化教研室,河北 张家口〓075000 
徐志伟 河北北方学院检验系生化教研室,河北 张家口〓075000 
摘要点击次数: 1202
全文下载次数: 209
中文摘要:
      目的:观察维生素A(vitamin A,Vit A)结合胰岛素干预对糖尿病大鼠血糖影响?方法:以40 mg/kg 剂量链脲佐菌素(streptozotocin,STZ)诱导Wistar大鼠轻度糖尿病并随机分为模型组?胰岛素治疗组?单纯Vit A治疗组?Vit A联合胰岛素治疗组,另取正常小鼠作为正常组,每组10只?胰岛素每日2次皮下注射,剂量均为20 U/(kg·d),Vit A每天2次灌胃,剂量为80 000 U/(kg·d)?干预前及干预间,高效液相色谱法测定血清视黄醇;干预结束后分析空腹血糖和腹腔糖耐量;取胰腺,免疫组化染色,图像分析系统分析β细胞总量;在含或不含视黄酸条件下体外培养正常大鼠胰岛,CCK-8处理后,酶标仪分析视黄酸对胰岛细胞增殖的影响?结果:Vit A和胰岛素干预停止后的2?4?6天,胰岛素治疗组及单纯Vit A治疗组空腹血糖与模型组相比均无统计学差异,Vit A联合胰岛素治疗组空腹血糖[分别为(6.68±1.82)?(7.14±1.27)?(6.82±1.36)mmol/L],糖耐量曲线下面积(19.96±4.76)mmol/L与模型组相比均存在统计学差异(P < 0.05);胰岛素治疗组及单纯Vit A治疗组β细胞总量分别为(1.78±0.31)?(1.67±0.85)mg,与模型组(1.82±0.25)mg相比均无统计学差异,Vit A联合胰岛素治疗组为(3.25±0.73)mg,与模型组相比显著增加(P < 0.05);视黄酸不影响体外胰岛细胞增殖?结论:Vit A结合胰岛素治疗使STZ糖尿病大鼠胰岛β细胞总量增加,空腹血糖和糖耐量趋于正常?
英文摘要:
      Objective:To explore effect of combination therapy with vitamin A(Vit A) and insulin on blood glucose in dibetic rats. Methods:Mild diabetes mellitus was induced in male Wistar rats by a single intraperitoneal injection of streptozotocin(STZ) at a dose of 40 mg/kg. Diabetic rats were assigned randomly to the model group,insulin treatment group,Vit A treatment group,Vit A combined with insulin treatment group,and normal rats were used as the control group(n=10 in each group). Insulin treatment was accomplished by subcutaneous injection twice daily for 3 days at a dose of 20 U/(kg·d). Vit A treatment was accomplished by gastric perfusion twice daily for 3 days at a dose of 80 000 U/(kg·d). Serum retinol in various groups was measured by high performance liquid chromatography before and during the interference. Fasting blood glucose and intraperitoneal glucose tolerance test in 5 groups were analyzed at different times following the interference. The pancreas tissue were obtained from 5 groups of rats,and stained immunohistochemically for β cells mass measurement using an image analysis system. Islet isolated and purified from normal rats were cultured with or without retinoic acid. The proliferation of islet cells treated was measured by CCK-8 method. Results:At 2 day,4 day and 6 day following the interference,fasting blood glucose in insulin treatment group and Vit A treatment group had no significant difference compared with that in diabetic model group. And the fasting blood glucose in Vit A combined with insulin treatment group were (6.68±1.82)mmol/L,(7.14±1.27)mmol/L,(6.82±1.36)mmol/L at 2,4 and 6 day of treatment respectively,which were significantly different from those in diabetic model group(P < 0.05). The area under the curve of glucose tolerance in Vit A combined with insulin treatment group was(19.96±4.76)mmol/L,which was significantly different from (42.26±4.38)mmol/L in diabetic model group (P < 0.05). Islet β cells mass in insulin treatment group and Vit A treatment group were(1.78±0.31)mg and(1.67±0.85)mg respectively,which were no significance compared with(1.82±0.25)mg in diabetic model group,and the index in Vit A combined with insulin treatment group was (3.25±0.73)mg,which was significantly higher than that of diabetic model group(P < 0.05). In vitro islet culture showed that retinoic acid did not affect islet cell proliferation. Conclusion:Combination therapy with vitamin A and insulin can normalize fasting blood glucose and glucose tolerance in STZ diabetic rats through increasing Β cell mass.
查看全文   查看/发表评论  下载PDF阅读器
关闭