文章摘要
张〓颖,何帮顺,潘玉琴,刘长明,王书奎.白细胞介素10基因启动子区多态性与胃癌易感性的关系研究[J].南京医科大学学报,2010,(9):1295~1300
白细胞介素10基因启动子区多态性与胃癌易感性的关系研究
Study on the relationship between polymorphism sites of IL-10 and the susceptibility of gastric cancer
投稿时间:2010-04-22  
DOI:10.7655
中文关键词: 胃癌  疾病遗传易感性  白细胞介素10  多态性  限制性片段长度
英文关键词: gastric cancer  genetic susceptibility  interleukin-10  polymorphism  restriction fragment length
基金项目:南京医科大学科技发展基金面上项目(08NMUM107)
作者单位
张〓颖 南京医科大学附属南京第一医院中心实验室,江苏 南京〓210006 
何帮顺 南京医科大学附属南京第一医院中心实验室,江苏 南京〓210006 
潘玉琴 南京医科大学附属南京第一医院中心实验室,江苏 南京〓210006 
刘长明 昆山市中医院检验科,江苏 昆山 215300 
王书奎 南京医科大学附属南京第一医院中心实验室,江苏 南京〓210006 
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中文摘要:
      目的:分析胃癌(gastric cancer,GC)患者白细胞介素10(Interleukin-10,IL-10)的启动子区域转录起始位点上游第1082位点?819位点及592位点多态性,探讨其与GC易感性之间的关系?方法:应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测GC组患者129例和正常对照组98例健康人的IL-10的基因多态性,免疫印迹法检测GC组与对照组的幽门螺旋杆菌(H.pylori)感染情况,SPSS11.0软件包统计分析各位点的基因型分布和等位基因频率?结果:IL-10-1082位点的多态性在GC组(AA:57.36%?GA:40.31%?GG:2.32%)与对照组(AA:75.51%?GA:24.49%)的基因频率有显著性差异(P=0.01)?GA基因型在GC组中的频率显著高于对照组(P=0.012),G等位基因在GC组中的频率显著高于对照组(P=0.007)?IL-10-819及IL-10-592的各基因型及其等位基因在GC组及对照组中分布均无显著性差异(P > 0.05)?在H.pylori感染组中IL-10-1082GA基因型在GC组中的分布显著高于其在对照组中的分布(OR=2.65,95% CI 1.16,6.08;P=0.031)?结论:IL-10-1082 GA基因型携带者GC的发病风险增高,IL-10-1082位点的G等位基因发病风险增高,且IL-10-1082 GA基因型与H.pylori感染对于GC的发生有协同作用?
英文摘要:
      Objective:To explore the relationship of the gastric cancer susceptibility and polymorphism of upstream of IL-10 promoter at the site of 1082,819 and 592. Methods:Total of 129 patients with gastric cancer and 98 healthy individuals were enrolled in the study,and divided into patient group and control group. The polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) was applied to detect the base transition in the upstream of IL-10 promoter at the sites of 1082,819 and 592. The H.pylori infection of patients and health individuals was detected by immunoblotting. The SPSS10.0 software was applied to analyze frequencies of all genotypes. Results:There were obviously differences in polymorphism of IL-10-1082 between the gastric cancer group(AA:57.36%,GA:40.31% and GG:2.32%)and control group(AA:75.51%,GA:24.49%). The rate of GA genotype of IL-10-1082 in gastric cancer group was higher than that in control group(P=0.012). Allele G of IL-10-1082 in gastric cancer group was also higher than that in control group(P=0.007). We failed to observe any difference in the genotypes of IL-10-819 and -592 between gastric cancer group and control group. The GA genotype of IL-10-1082 in gastric cancer group was higher than that in the individuals with H.pylori infection in control group(OR=2.65,95% CI:1.16-6.08;P=0.031). Conclusion:The GA genotype or G allele in IL-10-1082 is a risk factor of the occurrence of gastric cancer. There was cooperation of H.pylori infection and the GA genotype in IL-10-1082 on the occurrence of GC.
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