文章摘要
张 梅,徐书杭,徐 瑜,茅晓东,刘翠萍,徐宽枫,刘 超.抗原特异性CD4+CD25+ T细胞的制备及功能鉴定[J].南京医科大学学报,2010,(11):1590~1593
抗原特异性CD4+CD25+ T细胞的制备及功能鉴定
Induction and identification of allogeneic antigen-specific CD4+CD25+ regulatory T cells
投稿时间:2010-04-29  
DOI:10.7655
中文关键词: CD4+CD25+调节性T细胞  同种异体抗原  无能  抑制  γ干扰素
英文关键词: CD4+CD25+ regulatory T cells  alloantigen  anergy  suppression  IFN-γ
基金项目:江苏省政府医学重点学科项目基金(苏卫科教2001-34)
作者单位
张 梅 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
徐书杭 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
徐 瑜 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
茅晓东 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
刘翠萍 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
徐宽枫 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
刘 超 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
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中文摘要:
      目的: 制备供体抗原特异性CD4+CD25+调节性T细胞,并对诱导的细胞进行免疫生物学功能鉴定?方法:分离供体ICR小鼠的脾细胞经尾静脉免疫BALB/cByJ小鼠,每周1次,连续3周?第4周,体外免疫磁珠分离技术(MACS)分选被免疫小鼠CD4+CD25+ T细胞和CD4+CD25-T细胞?混合淋巴细胞反应和ELISA检测细胞的功能?结果:体外共培养实验显示,被免疫BALB/cByJ小鼠的脾细胞呈低增殖反应?CD4+CD25+T细胞能够抑制同种异体抗原刺激的天然(na?觙ve)BALB/cByJ小鼠CD4+CD25-T细胞的增殖反应和γ干扰素(IFN-γ)的分泌?结论:供体特异性脾细胞输注后分选的CD4+CD25+调节性T细胞是抗原特异性的,在体外具有免疫无能性和免疫抑制性?
英文摘要:
      Objective: To induce allogeneic antigen-specific CD4+CD25+ regulatory T cells in vivo and identify the function of these cells. Methods: Allogeneic antigen-specific CD4+CD25+ regulatory T cells were generated by the addition of multiple intravenous injections of ICR mice splenocytes in vivo. Spleen cells were then pooled and enriched for CD4+CD25+ T cells and CD4+CD25- T cells by negative and positive selection using MACS in vitro. Characterizations of allogeneic antigen-specific CD4+CD25+ regulatory T cells were tested by mixed lymphocyte reaction(MLR) consisting of BALB/cByJ splenocytes as responder and MMC-treated ICR splenocytes as stimulator. The abilities of these cells to respond to various stimulators and to suppress alloreactive T cells responses were tested in vitro. Results: In the MLR, allogeneic antigen-specific T cells displayed little proliferation with allogeneic-antigen existence. Furthermore, these cells inhibited the proliferative responses and IFN-γ production of na?觙ve CD4+CD25- T cells to alloantigens in vitro. Conclusion: Donor-specific splenocyte transfusion-induced CD4+CD25+ T cells were allogeneic antigen-specific. They were anergic,and retained their ability to suppress antigen-driven responses of CD4+CD25- T cells in vitro.
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