文章摘要
王 彬,张爱青,龚 晶,郑 君,曾智凤,甘卫华.蟾蜍灵调控系膜细胞增殖与细胞周期激酶抑制剂p21的关系[J].南京医科大学学报,2010,(12):1696~1699
蟾蜍灵调控系膜细胞增殖与细胞周期激酶抑制剂p21的关系
Role of cyclin kinase inhibitor p21 in the regulation of bufalin on mesangial cell proliferation
投稿时间:2010-05-28  
DOI:10.7655
中文关键词: 蟾蜍灵  系膜细胞  p21
英文关键词: bufalin  mesangial cell  p21
基金项目:江苏省“六大人才高峰”项目资助(06-B-020)
作者单位
王 彬 南京医科大学第二附属医院儿肾科,江苏 南京 210003 
张爱青 南京医科大学第二附属医院儿肾科,江苏 南京 210003 
龚 晶 南京医科大学第二附属医院儿肾科,江苏 南京 210003 
郑 君 南京医科大学第二附属医院儿肾科,江苏 南京 210003 
曾智凤 南京医科大学第二附属医院儿肾科,江苏 南京 210003 
甘卫华 南京医科大学第二附属医院儿肾科,江苏 南京 210003 
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中文摘要:
      目的: 观察蟾蜍灵对大鼠系膜细胞(mesangial cell,MC) p21表达的影响,探讨其调控系膜细胞增殖的机制?方法:MTT法观察不同浓度蟾蜍灵(0.01?0.04?0.08?0.16 μmol/L)对PDGF-BB(20 ng/ml)诱导的MC 增殖的影响;流式细胞术测定MC细胞周期的变化;RT-PCR法测定p21基因表达的改变;Western blot法测定p21蛋白水平变化?结果:PDGF-BB刺激MC12 h后,加入不同浓度蟾蜍灵干预24 h,蟾蜍灵从0.04 μmol/L至0.16 μmol/L的呈浓度依赖抑制PDGF-BB诱导的MC增殖(P < 0.05)?0.08 μmol/L蟾蜍灵干预24 h后,可使PDGF-BB诱导的MC G0/G1期细胞比例升高,使S期细胞比例下降,且上调P21mRNA和蛋白水平的表达(P < 0.05)?0.08 μmol/L蟾蜍灵对正常系膜细胞无明显影响(P > 0.0.5)?结论:蟾蜍灵可能通过上调细胞周期激酶抑制剂p21的表达来抑制PDGF-BB诱导的MC的增殖?
英文摘要:
      Objective: To investigate the role of p21 in the regulation of bufalin on the mesangial cell (MC) proliferation. Methods: The effects of different concentrations of bufalin (0.01,0.04,0.08,0.16 μmol/L) on MC proliferation induced by PDGF-BB(20 ng/ml) was evaluated by MTT test; Flow cytometry and RT-PCR were utilized for cell cycle analysis and p21 DNA expression,,respectively. Western blot was used to analyze protein expression of p21. Results: After twelve hours of PDGF-BB stimulation, MC was cultured with different concentrations of bufalin from 0.04 μmol/L to 0.16 μmol/L for 24 hours, respectively. Proliferation of GMC induced by 20 ng/ml PDGF-BB was inhibited significantly by bufalin in a dose-dependent manner(P < 0.05). Similar results were observed in 0.08 μmol/L bufalin, which blocked the PDGF-BB-induced progression from G0/G1 to S phase of the cell cycle in synchronized cells(P < 0.05). In addition, we found that bufalin reversed PDGF-BB-induced down-regulation of cyclin-dependent kinase inhibitor p21 on both gene and protein level(P < 0.05). However, 0.08μmol/L bufalin had no effects on normal mesangial cell proliferation (P > 0.05). Conclusion: Bufalin may inhibit the PDGF-BB-induced MC proliferation by increase of p21 expression.
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