文章摘要
吴婴南,倪 杰,卢 春,秦 娣,朱 玲.卡波济肉瘤相关疱疹病毒体外感染人牙龈成纤维细胞初探[J].南京医科大学学报,2011,(2):138~142
卡波济肉瘤相关疱疹病毒体外感染人牙龈成纤维细胞初探
Preliminary study on infection of human gingival fibroblast cells with Kaposi's sarcoma-associated herpesvirus in vitro
投稿时间:2010-07-14  
DOI:10.7655
中文关键词: 口腔卡波济肉瘤  卡波济肉瘤相关疱疹病毒  人牙龈成纤维细胞  感染
英文关键词: Oral KS  Kaposi’s sarcoma-associated herpesvirus  human gingival fibroblast cells  infection
基金项目:南京市人事局留学回国人员科研活动启动资金(1993-23);国家自然科学基金(30900064)
作者单位
吴婴南 南京医科大学附属口腔医院儿童牙病科,口腔医学研究所, 江苏 南京 210029 
倪 杰 南京医科大学附属口腔医院牙周科,江苏 南京 210029 
卢 春 南京医科大学微生物学与免疫学系,江苏 南京 210029 
秦 娣 南京医科大学微生物学与免疫学系,江苏 南京 210029 
朱 玲 南京医科大学附属口腔医院儿童牙病科,口腔医学研究所, 江苏 南京 210029 
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中文摘要:
      目的: 探索卡波济肉瘤相关疱疹病毒(Kaposi's sarcoma-associated herpesvirus,KSHV)体外感染人牙龈成纤维细胞(human gingival fibroblast,HGF)的途径和方法,为研究KSHV导致口腔卡波济肉瘤病变的机制提供依据?方法:用12-O-十四烷酰佛波醇-13-乙酯(12-O-tetradecanoylphorbol-13-acetate,TPA)刺激人原发性渗出性淋巴瘤(primary effusion lymphoma,PEL)细胞系的BC-3细胞,收集细胞上清(含KSHV病毒颗粒),感染HGF细胞?观察细胞病变效应(cytopatric effect,CPE),采用RT-PCR和Western blot分别检测HGF细胞内KSHV编码基因的转录情况和蛋白表达水平?结果:KSHV感染HGF细胞后可出现CPE;感染后采用RT-PCR可检测到不同时间点ORF26和ORF73基因的转录;感染后12 h可检测到vIL-6蛋白的表达?结论:KSHV可感染HGF细胞并建立潜伏感染,为进一步研究KSHV导致的口腔KS发病机制提供了较好的体外模型?
英文摘要:
      Objective: To establish a model of infecting human gingival fibroblast(HGF)cells with KSHV in vitro for further studying the mechanism of oral KS pathogenesy. Methods:The supernatants containing KSHV virion harvested from primary effusion lymphoma(PEL) cells BC-3 were used to infect HGF cells. The cytopathic effect (CPE) of HGF cells was observed post-infection and the lytic replication of KSHV in HGF cells was detected by RT-PCR and Western blot. Results:CPE appeared in post-infective HGF cells. KSHV ORF26 and ORF73 mRNA transcription was detected in HGF cells by RT-PCR at diffenrent time points. And the specific band of vIL-6 protein was detected at 12 hours post-infection by Western blot. Conclusion:KSHV could infect HGF cells and establish latent infection,providing a model in vitro for further studying the mechanism in KSHV leading to oral KS.
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