文章摘要
王 亮,尹 亮,蒋维维,刘养岁,孔连宝.基质金属蛋白酶9对大鼠肝脏缺血再灌注后caspase-3表达的影响[J].南京医科大学学报,2011,(2):175~178
基质金属蛋白酶9对大鼠肝脏缺血再灌注后caspase-3表达的影响
The effect of matrix metalloproteinase-9 on the expression of caspase-3 in hepatic ischemic reperfusion injury
投稿时间:2010-10-19  
DOI:10.7655
中文关键词: 强力霉素  基质金属蛋白酶  caspase-3  肝脏  缺血再灌注损伤
英文关键词: doxycycline  matrix metalloproteinase  caspase-3  liver  ischemia-reperfusion
基金项目:江苏省“六大人才高峰”(第五批高层次人才项目)
作者单位
王 亮 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
尹 亮 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
蒋维维 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
刘养岁 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
孔连宝 南京医科大学第一附属医院肝脏外科,江苏 南京 210029 
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中文摘要:
      目的:探讨基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)对SD大鼠肝脏缺血再灌注后caspase-3表达的影响及其可能的机制?方法:建立雄性SD大鼠70%肝脏缺血再灌注损伤模型?将48只大鼠随机分为3组(n=16):假手术组(S组),缺血再灌注组(I/R组)和强力霉素处理组(DC组)?再灌注3 h 后检测血清中丙氨酸转氨酶(ALT) ?天冬氨酸转氨酶(AST)?肿瘤坏死因子-α(TNF-α)?肝组织丙二醛(MDA)含量?通过免疫组化方法检测肝脏组织中MMP-9及caspase-3的表达情况?结果:肝脏缺血再灌注后,IR 组的ALT?AST? TNF-α?MDA的阳性表达率明显高于S 组(P < 0.05),而DC组中各指标得到改善?在I/R组中,MMP-9及caspase-3较S组表达增强,而在DC组中其表达较I/R组减弱?结论:MMP-9的低表达,可减轻炎症细胞及因子的释放和浸润,抑制caspase-3介导的肝脏细胞的凋亡?
英文摘要:
      Objective:To investigate the effect of matrix metalloproteinase-9 on the expression of caspase-3 and its possible mechanisms. Methods:The model of 70% hepatic ischemia-reperfusion was established in SD male rat. Total 48 SD rats were randomly divided into three groups(n = 16): sham operation group(S group),ischemia-reperfusion group(I/R group) and doxycycline treated group(DC group). After 3 hours of reperfusion,the enzyme levels in serum were examined, including ALT,AST,TNF-α and MDA. The expressions of MMP-9 and Caspase-3 were determined in immunohistochemistry. Results:After ischemia-reperfusion,the levels of ALT,AST,TNF-α and MDA expression in I/R group was significantly higher than those in S group. After treatment of oral doxycycline,the all above abnormal parameters were retrieved remarkably(P < 0.05). In the I/R group,MMP-9 and caspase-3 expressions increased,comparing with that in S group,while its expression decreased in the DC group,comparing with that in I/R group. Conclusion:The low expression of MMP-9 reduces the release and infiltration of inflammatory cells,inhibits caspase-3-mediated apoptosis of liver cells.
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