文章摘要
华 烨,丁新生,孔岳南.细胞外信号调节激酶对氧糖剥夺后大鼠海马神经元的作用研究[J].南京医科大学学报,2011,(2):185~189
细胞外信号调节激酶对氧糖剥夺后大鼠海马神经元的作用研究
Effect of extracellular signal regulated kinase on cultured hippocampal neurons of rats with oxygen-glucose deprivation
投稿时间:2010-08-03  
DOI:10.7655
中文关键词: 细胞外信号调节激酶  氧糖剥夺  海马神经元  凋亡
英文关键词: excelluar signal-regulated kinase  oxygen-glucose deprivation  hippocampal neuron  apoptosis
基金项目:
作者单位
华 烨 南京医科大学附属无锡二院神经内科,江苏 无锡 214002 
丁新生 南京医科大学第一附属医院神经内科,江苏 南京 210029 
孔岳南 南京医科大学附属无锡二院神经内科,江苏 无锡 214002 
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中文摘要:
      目的: 研究细胞外信号调节激酶对氧糖剥夺(oxygen-glucose deprivation,OGD)后大鼠海马神经元的作用? 方法:建立培养乳鼠海马神经元OGD模型,并分为正常对照组?OGD组?PD 98059 10 μmol/L?30 μmol/L组?流式细胞仪Annexin V/PI双染色法检测神经元细胞凋亡率,Western blot法检测ERK1/2?pERK1/2的表达? 结果: 与正常对照组相比,OGD组神经元的凋亡率升高(P < 0.01),pERK1/2的表达降低(P < 0.01),与OGD组相比,PD98059组神经元凋亡率明显升高(P < 0.01),pERK1/2的表达明显降低(P < 0.01), 30 μmol/L组较10 μmol/L组凋亡率升高及pERK1/2表达降低更为显著(P < 0.01),各组ERK含量无明显变化(P > 0.05)?结论: ERK可能参与氧糖剥夺后的神经元凋亡,抑制ERK通路可促进神经元凋亡?
英文摘要:
      Objective: To investigate the effect of excelluar signal-regulated kinase(ERK) on rat hippocampal neurons with oxygen-glucose deprivation(OGD). Methods:Cultured hippocampal neurons were designed into four groups:normal group,OGD group, PD98059 10 μmol/L and 30 μmol/L groups. The apoptosis of neurons was determined by flow cytometry, and Western blot was used to evaluate protein expression of ERK1/2 and pERK1/2. Results:Compared with normal control group, neuron apoptosis increased while pERK1/2 protein expression decreased obviously in OGD group(both P < 0.01). Neuron apoptosis increased and pERK1/2 protein expression decreased more significantly in PD98059 groups compared with OGD group(both P < 0.01). Changes in 30 μmol/L PD98059 group were more significant than 10 μmol/L PD98059 group (P < 0.01). Each group showed no significant change in ERK expression(P > 0.05). Conclusion:ERK may be involved in neuronal apoptosis after OGD, and inhibition of ERK pathway can promote neuronal apoptosis.
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