文章摘要
朱 婵,何帮顺,潘玉琴,徐勇飞,王书奎.shrna抑制cd147表达对喉癌细胞株hep2增殖和侵袭能力的影响[J].南京医科大学学报,2011,(3):351~355
shrna抑制cd147表达对喉癌细胞株hep2增殖和侵袭能力的影响
Effects of shrna on cd147 expression, proliferation and invasion in hep2 cells
投稿时间:2010-08-07  
DOI:10.7655
中文关键词: cd147  rna干扰  喉癌  细胞增殖
英文关键词: cd147  rna interference  laryngeal carcinoma  cell proliferation
基金项目:南京医科大学科技发展基金面上项目(08NMUM107)
作者单位
朱 婵 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
何帮顺 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
潘玉琴 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
徐勇飞 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
王书奎 南京医科大学附属南京第一医院中心实验室,江苏 南京 210006 
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中文摘要:
      目的:研究shrna抑制喉癌细胞株hep2中cd147表达对喉癌细胞增殖及侵袭能力的影响?方法:将前期构建的重组质粒psilencer-shrna1和psilencer-shrna2分别转染至喉癌细胞hep2中,同时以转染空载质粒psilencer-shrna-control和空白细胞作为对照,g418筛选稳定转染细胞系?筛选后的4组细胞分别命名为hep2?hep2/shrna-control?hep2/shrna1和 hep2/shrna2?实时荧光定量pcr法和western blot检测cd147的表达?采用mtt法检测4组细胞增殖能力?采用transwell法检测4组细胞的体外侵袭能力?结果:转染后的喉癌细胞株hep2/shrna1和 hep2/shrna2中cd147的mrna和蛋白表达水平与hep2相比显著下调,细胞增殖能力分别下降到67.49%(p < 0.01)和61.37%(p < 0.01),侵袭能力也分别下降了61.80%(p < 0.01)和73.58%(p < 0.01)?结论:cd147 shrna能下调喉癌细胞株hep2中cd147的表达,抑制喉癌细胞hep2的增殖和侵袭能力?
英文摘要:
      objective:to investigate the effect of silencing cd147 expression on proliferation and invasion of human laryngeal carcinoma cell line hep2. methods:previously prepared recombinant plasmids psilencer-shrna-control, psilencer-shrna1 and psilencer-shrna2 were transfected into the hep2 cell. the rna interference efficiency of cd147 was determined by real-time pcr and western blot. the proliferation was measured by mtt assay. the transwell test was used to detect the invasion ability of the hep2 cells, and psilencer-shrna-control transfected hep2 and normal hep2 were used as control groups. results:after the transfection with psilencer-shrna1 and psilencer-shrna2, the mrna and protein levels of cd147 in the hep2 cells were both significantly down-regulated. proliferation was decreased significantly to 67.49%(p < 0.01)and 61.37%(p < 0.01),respectively. their invasive ability were also significantly decreased to 61.80%(p < 0.01)and 73.58%(p < 0.01),respectively. conclusion:the shrna down-regulated the expression of cd147, inhibited the proliferation and invasive ability of hep2 cells.
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