文章摘要
陈仁杰,马 俊,张大为,毛 圆,朱 进,冯振卿.鼻息肉患者血清抗ebv-lmp1抗体的检测及其临床意义[J].南京医科大学学报,2011,(4):526~528
鼻息肉患者血清抗ebv-lmp1抗体的检测及其临床意义
detection and significance of anti-ebv-lmp1 antibody in patients with nasal polyps
投稿时间:2010-08-15  
DOI:10.7655
中文关键词: 鼻息肉  epstain-barr病毒  潜伏膜蛋白1  抗体  检测
英文关键词: nasal polyp  epstain-barr virus  latent membrane protein1  antibody  detection
基金项目:江苏省基础研究计划(自然科学基金)(BK2008481);南京医科大学科技发展基金重点项目(NY2005D2D13);江苏省科技厅社会发展(支撑计划)项目(BE2009152)
作者单位
陈仁杰 南京医科大学第二附属医院耳鼻喉-头颈外科,江苏 南京 210011 
马 俊 南京医科大学第二附属医院耳鼻喉-头颈外科,江苏 南京 210011 
张大为 南京医科大学第二附属医院耳鼻喉-头颈外科,江苏 南京 210011 
毛 圆 南京医科大学第二附属医院耳鼻喉-头颈外科,江苏 南京 210011 
朱 进 南京医科大学卫生部抗体技术重点实验室,江苏 南京 210029 
冯振卿 南京医科大学卫生部抗体技术重点实验室,江苏 南京 210029 
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中文摘要:
      目的: 检测鼻息肉患者血清抗epstain-barr病毒(ebv)潜伏膜蛋白1(lmp1)抗体并探讨其临床意义?方法:行功能性鼻内镜手术治疗的鼻息肉患者20例为实验组,健康自愿者20例为对照组?以谷胱甘肽-ebv-lmp1胞外区融合蛋白(gst-lmp1)为检测抗原,elisa 和western blot检测健康自愿者和鼻息肉患者手术治疗前?治疗后第15?30?90?180天血清抗ebv-lmp1抗体?结果:elisa检测鼻息肉患者和健康自愿者血清抗ebv-lmp1抗体水平分别为0.976±0.131?0.400±0.102,两组比较有统计学差异(p < 0.01)?鼻息肉患者术前和术后第15?30?90?180天血清抗ebv-lmp1抗体水平分别为:0.976±0.131?0.921±0.138?0.899±0.168?0.576±0.144?0.521±0.117,术后第90?180天与术前比较,差异有统计学意义(p < 0.01);但是术后第15?30天与术前比较,无统计学差异?western blot 检测鼻息肉患者术前血清抗体水平与术后第15?30天比较无统计学差异;但是与术后第90?180天比较有统计学差异(p < 0.01)?结论:鼻息肉和ebv感染有关,鼻息肉患者血清抗ebv-lmp1抗体水平随着疾病的有效治疗而下降,可作为评价临床疗效的有效指标?
英文摘要:
      objective:to detect the serum epstain-barr virus(ebv)latent membrane protein 1(lmp1)antibody and to appraise its clinical significance in patients with nasal polyps. methods:there were 20 patients with nasal polyps who were managed by functional endoscopic sinus surgery(fess) in experimental group,20 cases of healthy volunteers in control group. the anti-lmp1 antibody in sera of two groups before operation and 15,30,90,180 days after operation were detected by elisa and western blot using glutathione-ebv-lmp1 extracellular domain fusion protein(gst-lmp1)as the antigen. results:the average levels of serum anti-ebv-lmp1 antibody displayed by absorbance of elisa in nasal polyps group and control group were significantly different(0.976±0.131 vs. 0.400±0.102,p < 0.01). the levels of anti-ebv-lmp1 antibody in the patients with nasal polyps before operation and 15,30,90 and 180 days after surgery were 0.976±0.131,0.921±0.138,0.899±0.168,0.576±0.144 and 0.521±0.117,respectively. the anti-ebv-lmp1 levels in sera of patients with nasal polyp decreased significantly in postoperative day 90 and 180 compared with those before operation(p < 0.01),but there was no significant difference among the levels before operation and in postoperative day 15 and 30. western blot also showed the same changes. conclusion:nasal polyp may be closely related to ebv infection,the serum anti-lmp1 antibody level in the patients with nasal polyp declined following effective treatment,and it may be used as a valid indicator of clinical therapeutic efficacy.
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