HIF-1α?EphA2表达对食管鳞癌细胞体外三维培养的影响
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国家自然科学基金资助项目(30770991,30800511)


Effect of expression of HIF-1α and EphA2 on vasculogenic mimicry in carcinoma
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    摘要:

    目的:探讨肿瘤细胞缺氧诱导因子1α(HIF-1α)-上皮细胞激酶(EphA2)对食管鳞癌血管生成拟态(vasculogenic mimicry,VM)的影响及两者之间的关系-方法:HIF-1α-EphA2干扰质粒分别转染食管癌Eca109细胞和TE13细胞;RT-PCR-Western blot分别检测转染细胞中HIF-1α-EphA2的抑制效率;体外三维培养模拟血管生成拟态模型观察HIF-1α-EphA2抑制后细胞管状结构数量变化-结果: HIF-1α表达抑制伴随有EphA2表达下调,而EphA2表达抑制对HIF-1α表达无明显影响-HIF-1α-EphA2 miRNA干扰质粒能有效抑制Eca109和TE13中HIF-1α-EphA2的mRNA表达,无论HIF-1α或EphA2表达抑制,均能导致三维培养中管状结构数量下降-但HIF-1α抑制对管状结构的影响要强于EphA2-结论: HIF-1α-EphA2与食管癌血管生成拟态密切相关-EphA2表达调控可能是HIF-1α参与肿瘤细胞VM的形成机制之一,此外还可能存在其他调控途径-

    Abstract:

    Objective: To investigate the role and the correlation of HIF-1α and EphA2 in the process of vasculogenic mimicry in esophageal squamous cell carcinoma. Methods: Two esophageal cancer cell lines Eca109 and TE13 were transfected by plasmid harboring interfering RNA targeting HIF-1α and EphA2. The relationship of the expression of HIF-1α and EphA2 was measured by RT-PCR and Western blot. The formation of tubular networks of Eca109 and TE13 cells was analyzed by three-dimension culture in vitro.Results: ①The expression of EphA2 in Eca109 and TE13 was inhibited with silencing HIF-1α. On the contrary, the expression of HIF-1α in Eca109 and TE13 was not obviously inhibited with silencing EphA2. ②The mRNA expression of HIF-1α and EphA2 were markedly inhibited in Eca109 and TE13 lines with silencing HIF-1α and EphA2 gene. ③The numbers of tubular networks were both remarkably decreased in Eca109 and TE13 with silencing HIF-1α and EphA2 gene, but the number of the former was more than the latter. Conclusion: HIF-1α and EphA2 may both participate the formation of vasculogenic mimicry of esophageal squamous cell carcinoma. EphA2 may be one of the important factors regulated by HIF-1αin the process.

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王 频,王 璐,丁宗励,曾楷峰,金海林,朱 宏,施瑞华. HIF-1α?EphA2表达对食管鳞癌细胞体外三维培养的影响[J].南京医科大学学报(自然科学版),2011,(6):783-788

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  • 收稿日期:2010-12-21
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