文章摘要
刘 玉,杨 洁,孙卫斌.分泌性hrCEMP1在NIH3T3细胞中的表达[J].南京医科大学学报,2011,(6):799~802
分泌性hrCEMP1在NIH3T3细胞中的表达
Expression of human cementum protein 1 in cultured NIH3T3 cell
投稿时间:2010-11-24  
DOI:10.7655
中文关键词: hrCEMP1  NIH3T3细胞株  基因转染
英文关键词: human cementum protein 1  NIH3T3 cell line  gene transfection
基金项目:江苏省自然科学基金项目(BK2010118);南京市医学科技发展重点项目(ZKX08017);南京市医学科技发展一般性课题(YKK10126)
作者单位
刘 玉 南京大学口腔医学院,江苏 南京 210008 
杨 洁 南京大学口腔医学院,江苏 南京 210008 
孙卫斌 南京大学口腔医学院,江苏 南京 210008 
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中文摘要:
      目的:通过转基因技术建立稳定表达hrCEMP1的成纤维细胞株?方法:利用高效率的阳离子聚合物,将含有hrCEMP1编码序列的真核表达质粒pcDNA3.1-hrCEMP1转染入NIH3T3细胞中,用G418筛选获得稳定转染细胞株,以RT-PCR检测hrCEMP1基因转录,以酶联免疫吸附试验(ELISA)检测hrCEMP1蛋白表达?结果:转染细胞株内有hrCEMP mRNA的转录,并可检测到hrCEMP1蛋白的表达?结论:通过转基因手段,可以将外源性hrCEMP1基因成功导入成纤维细胞内,为进一步研究hrCEMP1对成纤维细胞的诱导作用建立基础?
英文摘要:
      Objective:To establish fibroblasts stably expressing human cementum protein 1 (hrCEMP1). Methods: Eukaryotic expression vector pcDNA3.1-hrCEMP1 was transduced into NIH3T3 cells using SofastTM,and stably transfecting cell line was selected by G418. The transcription of hrCEMP1 in the transfecting cells was determined by RT-PCR and enzyme-linked immunosorbent assay(ELISA). Results: hrCEMP1 mRNA was transcripted and expressed in the transfected NIH3T3 cells. Conclusion:With positive compound transfection, outside hrCEMP1 gene can be successfully transducted and expressed in NIH3T3 cells, which is the foundation of researching the induction of hrCEMP-1 on fibroblast cells.
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