文章摘要
朱 勤,王 强,王文娟,程 洁,高 蓉,肖 杭.代森锰锌对小鼠生精细胞T型Ca2+通道的作用[J].南京医科大学学报,2011,(7):996~1001
代森锰锌对小鼠生精细胞T型Ca2+通道的作用
Effects of mancozeb on T-type calcium channels in mouse spermatogenic cells
投稿时间:2011-01-06  
DOI:10.7655
中文关键词: 代森锰锌  T型Ca2+通道  生精细胞  小鼠
英文关键词: mancozeb  T-type Ca2+ channel  spermatogenic cells  mouse
基金项目:国家自然科学基金项目(30771831,81072329)
作者单位
朱 勤 南京医科大学现代毒理学教育部重点实验室,江苏 南京 210029 
王 强 南京医科大学现代毒理学教育部重点实验室,江苏 南京 210029 
王文娟 南京医科大学现代毒理学教育部重点实验室,江苏 南京 210029 
程 洁 南京医科大学现代毒理学教育部重点实验室,江苏 南京 210029 
高 蓉 南京医科大学现代毒理学教育部重点实验室,江苏 南京 210029 
肖 杭 南京医科大学现代毒理学教育部重点实验室,江苏 南京 210029 
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中文摘要:
      目的:研究农药代森锰锌(mancozeb)对小鼠生精细胞T型钙电流(ICaT)的影响?方法:采用膜片钳全细胞记录技术观察代森锰锌对急性机械分离的小鼠生精细胞ICaT 的影响?结果:Mancozeb(10?20?40?80?120 μmol/L)呈浓度?电压依赖性抑制小鼠生精细胞钙电流,抑制率分别为(9.93±0.90)%?(20.93±2.50)%?(25.92±2.80)%?(42.64±3.10)%?(56.80±3.20)% (n=6,P<0.05)?Mancozeb 对T型钙通道的半数最大抑制浓度(K50)为35.60 μmol/L?120 μmol/L mancozeb显著改变T型Ca2+通道的激活和失活特性:半数激活电压(V1/2a)和激活斜率因子(κa)分别从(-47.09±1.05)mV和(8.67±0.78) mV变为(-51.46±0.84)mV和(10.56±0.619)mV (n=5, P < 0.05);而半数失活电压(V1/2i)和失活斜率因子(κi)分别从(-62.96±3.36)mV和(9.93±1.41)mV变为 (-64.11±5.55)mV和(13.64±1.95)mV (n=5,P < 0.05)?结论:Mancozeb对生精细胞T型钙通道有抑制作用,且呈浓度依赖性?
英文摘要:
      Objective:To investigate the effect of mancozeb on T-type calcium currents(ICaT) in spermatogenic cells. Methods: Ca2+ currents were obtained in acutely dissociated mouse spermatogenic cells by the whole-cell patch clamp technique and the effects of mancozeb on ICaT were observed. Results:Mancozeb at the concentrations of 10, 20, 40, 80 and 120 μmol/L significantly inhibited ICaT in the mouse spermatogenic cells, with the K50 value of 35.60 μmol/L and the inhibition rates of mancozeb were(9.93±0.90)%,(20.93±2.50)%,(25.92±2.80)%,(42.64±3.10)% and (56.80±3.20)%, respectively(n=6, P < 0.05). Mancozeb of 120 μmol/L significantly changed the activation and inactivation of ICaT: the half activation potential(V1/2a) and the activation steepness factor (κa) from (-47.09±1.05)mV and (8.67±0.78)mV to (-51.46±0.84)mV and (10.56±0.619)mV, respectively(n=5, P < 0.05); the half inactivation potential (V1/2i) and the inactivation steepness factor (κi) from (-62.96±3.36)mV and (9.93±1.41)mV to(-64.11±5.55)mV and (13.64±1.95)mV, respectively (n=5, P < 0.05). Conclusion:Mancozeb has significant inhibitory effects on ICaT in mouse spermatogenic cells with concentration dependence.
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