两种测定大鼠心肌缺血再灌注损伤后梗死面积方法的比较
DOI:
作者:
作者单位:

作者简介:

通讯作者:

中图分类号:

基金项目:

国家自然科学基金(81070275);国家基础研究项目973(2010CB535010)


Comparison of the two different methods of measuring infarct size after rats were subjected to ischemia and reperfusion
Author:
Affiliation:

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的:探索一种在体测定大鼠心肌缺血再灌注损伤后心肌梗死面积的方法,并与传统的离体染色方法进行了比较,确定其可行性-方法:结扎雄性成年Sprague-Dawley大鼠冠状动脉前降支,给予缺血30 min,再灌注6 h,用超声心动图评价大鼠的心功能,用生化分析仪测定血清中的肌酸激酶(creatine kinase,CK)活性-再灌注24 h后,用离体和在体两种方法进行TTC(triphenyltetrazolium chloride,2,3,5-氯化三苯基四氮唑)-Evans blue双染,比较两种方法的成功率-心梗面积/缺血区面积(infarct area/risk area,IA/RA)与血清CK活性的相关性-结果:在体染色法的IA/RA与离体染色法没有显著性差异,但成功率高-其IA/RA与血清CK活性的相关性高于离体染色法-结论:在体染色法与离体染色法相比,操作简易,能更准确地反映心肌缺血再灌注损伤的程度-

    Abstract:

    Objective:To develop a new method of measuring infarct size by staining hearts in vivo after ischemia-reperfusion,and compare with the traditional method of staining in vitro to determine its feasibility. Methods:Adult male Sprague-Dawley rats were subjected to 30 min myocardial ischemia and 6 h reperfusion by ligation of the left anterior descending coronary artery. Cardiac function was evaluated by echocardiography and serum creatine kinase(CK) activity was also measured by a biochemical analyzer. After reperfusion for 24 h,hearts were staining by triphenyltetrazolium chloride(TTC)-Evans blue in vitro or in vivo. Infarct area(IA)/ risk area (RA) was calculated. Finally we compared the success rate of the two methods,and made correlation analysis of the IA/RA with serum CK activity. Results:There was no significant difference in IA/RA staining by the two different methods. However,success rate of staining in vivo was significant higher than that of staining in vitro. Besides,compared with staining in vitro,there was a stronger correlation between IA/RA and CK activity by staining in vivo. Conclusion:The method of staining in vivo is much easier to operate,and it can evaluate the damage by myocardial ischemia-reperfusion injury more accurately than that of staining in vitro.

    参考文献
    相似文献
    引证文献
引用本文

王卓璎,张 雯,李晓珍,韩 艺,陈燕萍,鲁 翔,季 勇.两种测定大鼠心肌缺血再灌注损伤后梗死面积方法的比较[J].南京医科大学学报(自然科学版),2011,(8):1101-1105

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-04-07
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期:
  • 出版日期: