2-DG对体外培养人食管癌细胞周期及凋亡的影响
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江苏省教育厅自然科学基金资助(10KJB320007)


Effects of 2-Deoxy-D-glucose on the cell cycle and apoptosis in human esophageal cancer cells Eca109
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    摘要:

    目的:研究2-脱氧葡萄糖(2-Deoxy-D-glucose,2-DG)对体外培养人食管癌Eca109细胞周期及凋亡的影响-方法:建立体外低氧模型,实验分常氧组和低氧组,利用体外细胞培养及流式细胞分析术等方法,测定不同浓度的2-DG对Eca109细胞周期-凋亡和增殖指数(PI)的影响-结果:常氧条件下2-DG浓度为0 mmol/L-80 mmol/L作用食管癌细胞24 h后,G1期细胞的比例分别为(44.87 ± 0.99)%-(64.60 ± 2.17)%,凋亡细胞的比例分别为(3.90 ± 1.61)%-(7.23 ± 1.43)%;低氧条件下G1期细胞的比例分别为(54.23 ± 1.42)%-(87.74 ± 2.19)%,凋亡细胞的比例分别为(38.02 ± 1.42)%-(68.72 ± 1.47)%,两组间比较有统计学差异(P < 0.05)-结论:2-DG能剂量依赖性地导致细胞周期阻滞及细胞凋亡,使G1期细胞增多,同时伴有S期-G2/M期细胞比例的相应减少,且在低氧条件下作用更加明显-

    Abstract:

    Objective:To investigate the effects of 2-Deoxy- D-glucose (2-DG) on the cell cycle and apoptosis of human esophageal cancer(EC) Eca109 cells. Methods:At two different levels of O2(19% and 1%),treating with various concentrations (0,5,10, 20,40,80 mmol/L) of 2-DG for 24 h,the cell cycle and apoptosis were detected by flow cytometry. Results:After treatment with 2-DG at concentrations of 0 and 80 mmol/L 2-DG for 24 h,the proportion of G1 phase cells under 19% level of O2 were (44.87 ± 0.99)% and (64.60 ± 2.17)%,the apoptotic Eca109 cells reached (3.90 ± 1.61)% and (7.23 ± 1.43)%,respectively. While at 1% O2 level,the proportion of G1 phase cells were (54.23 ± 1.42)% and (87.74 ± 2.19)% (P < 0.05),and the apoptotic Eca109 cells reached (38.02 ± 1.42)% and (68.72 ± 1.47)% (P < 0.05),respectively. Conclusion:2-DG is able to significantly induce cell cycle arrest and apoptosis effectively in a dose-dependent manner, and under hypoxic conditions,Eca109 cells become more hypersensitized to 2-DG.

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徐 瑾,于莲珍,唐玉林,王海洋,吴雨洁,杨树平.2-DG对体外培养人食管癌细胞周期及凋亡的影响[J].南京医科大学学报(自然科学版),2011,(8):1164-1168

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  • 收稿日期:2011-04-27
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