血浆miR-191和miR-27a在胃癌诊断中的价值
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江苏省科技厅科技发展计划(社会发展)重点项目(BS2007011)


MiR-191 and miR-27a in plasma are promising novel biomarkers for detection of gastric cancer
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    目的:研究胃癌患者和健康者血浆中miRNA的表达差异,为胃癌提供新的早期miRNA诊断标志物-方法:分别收集既往未行放化疗的初治胃癌患者和健康者外周血浆-采用商品化的试剂盒提取血浆中的RNA,用反转录及实时定量PCR的方法检测7种miRNA(miR-21--27a--191--379--658--433--383)的含量-结果:miR-191和miR-27a在胃癌患者血浆中的表达较健康者升高(P < 0.001),miR-191的AUC(ROC曲线下面积)为0.939,区分胃癌患者和健康者的敏感性和特异性分别为86.1%和100%-miR-27a的AUC为0.881,其敏感性和特异性为83.33%和86.67%-结论:外周血浆中miR-191和miR-27a有作为胃癌早期诊断的标志物的潜力-

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    Objective:To study the different expression of miRNA in plasma between gastric cancer patients and healthy people for finding new biomarkers. Methods:The levels of 7 miRNAs (miR-21,-27a,-191,-379,-658,-433,-383) in plasma samples from patients of gastric cancer and healthy controls were measured using real-time quantitative RT-PCR. Results:miR-191 and miR-27a both elevated significantly in gastric cancer patients’ plasma(P < 0.001). miR-191 yields an AUC(the area under the ROC curve) of 0.939, with 86.1% sensitivity and 100% specificity in discriminating gastric cancer from controls. miR-27a yields an AUC of 0.881, with 83.33% sensitivity and 86.67% specificity. Conclusion:miR-191 and miR-27a are potential novel biomarkers in detection of gastric cancer.

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朱程君,张锦英,魏 娟,刘怡茜,束永前.血浆miR-191和miR-27a在胃癌诊断中的价值[J].南京医科大学学报(自然科学版),2011,(8):1173-1178

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  • 收稿日期:2011-03-17
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