白藜芦醇对胃腺癌细胞株增殖和凋亡的影响
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江苏省自然科学基金资助(BK2007005)


The effects of Resveratrol on the proliferation and apoptosis of gastric adenocarcinoma cell lines
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    摘要:

    目的:研究白藜芦醇对胃癌细胞株增殖和凋亡的影响-方法:不同浓度白藜芦醇(0~150 μmol/L)作用人胃腺癌细胞株BGC823(低分化)和SGC7901(中分化),CCK-8试剂盒检测细胞增殖,流式细胞仪检测细胞凋亡,免疫印迹检测凋亡相关蛋白survivin的表达-结果:白藜芦醇对胃癌细胞株增殖能力呈时间和浓度依赖性抑制,150 μmol/L白藜芦醇作用人胃癌细胞株BGC823和SGC7901 72 h后,早期和晚期凋亡细胞比率均明显增加,而survivin蛋白表达均明显下降(P < 0.05)-结论:白藜芦醇以浓度和时间依赖性模式抑制胃癌细胞的增殖能力和活性,诱导胃癌细胞株BGC823和SGC7901发生凋亡,其机制可能与下调survivin蛋白的表达有关-

    Abstract:

    Objective:To investigate the effects of Resveratrol on the proliferation and apoptosis of gastric cancer cell lines. Methods:Gastric cancer cell lines BGC823 and SGC7901 were treated with Resveratrol (0~150 μmol/L) for different time (24~72 h). The proliferation,apoptosis and survivin protein expression of gastric cancer cells were detected by the CCK-8 kit,flow cytometry and Western blot,respectively. Results:Resveratrol inhibited the proliferation of BGC823 and SGC7901 in dose-and time-dependant model. The early and late apoptosis rates of BGC823 and SGC7901 were highly increased by treatment of 150 μmol/L Resveratrol for 72 h,while Survivin protein expression decreased significantly (all P < 0.05). Conclusion:Resveratrol inhibits the proliferation and induces the apoptosis of BGC823 and SGC7901,in which the down-regulation of survivin protein may be involved.

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李运红,陈 敏,邹晓平,于成功,吕 瑛,张晓琦,徐肇敏.白藜芦醇对胃腺癌细胞株增殖和凋亡的影响[J].南京医科大学学报(自然科学版),2011,(8):1192-1195

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  • 收稿日期:2011-03-27
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