文章摘要
任 青,陈永利,黄光荣,周 希,胡 平.兔子宫内膜干细胞的鉴定[J].南京医科大学学报,2011,(9):1383~1387
兔子宫内膜干细胞的鉴定
Identification of the rabbit endometrial stem cell
投稿时间:2011-04-15  
DOI:10.7655
中文关键词: 兔子宫内膜  兔子宫内膜干细胞  上皮细胞  间质细胞
英文关键词: rabbit endometrium  rabbit endometrial stem cell  epithelial cell  stromal cell
基金项目:十堰市科技攻关项目(045D)
作者单位
任 青 湖北医药学院附属人民医院妇产科,湖北 十堰 442000 
陈永利 湖北医药学院附属人民医院妇产科,湖北 十堰 442000 
黄光荣 湖北医药学院附属人民医院妇产科,湖北 十堰 442000 
周 希 湖北医药学院附属人民医院妇产科,湖北 十堰 442000 
胡 平 湖北医药学院附属人民医院妇产科,湖北 十堰 442000 
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中文摘要:
      目的:研究兔子宫内膜是否存在干细胞?方法:流式细胞术检测兔子宫内膜中上皮细胞及间质细胞的特异性标记分子,干细胞培养基培养出富含干细胞的集落,运用RT-PCR方法检测分化前后相关分子的表达,MTT法检测集落细胞分化前后增殖能力变化,流式细胞术检测分化前后的相关分子表达变化及细胞的凋亡情况?结果:兔子宫内膜存在上皮细胞及间质细胞两种细胞,干细胞培养基可培养出富含干细胞的集落,且分化前的细胞具有更高的增殖潜能,表现出更多的干细胞源性,具有更低的细胞凋亡比例?结论:兔子宫内膜存在富含干细胞的集落,提示存在子宫内膜干细胞?
英文摘要:
      Objective:To explore whether stem cell was expressed in rabbit endometrium. Methods: The markers of epithelial cell and stromal cell were detected by fluorescence activated cell sorter(FACS);Endometrial cells were cultured in stem cell culture medium;Sox2 and Oct4 expression in the colonies and the differentiated cells were characterized by reverse transcriptase-polymerase chain reaction (RT-PCR);Apoptosis and cell proliferation were detected by FACS and 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT) method,respectively. Results: Rabbit endometrium contains epithelial cells and stromal cells,stem cells colonies can be cultured in stem cell culture medium,and the colonies with Oct4 and Sox2 expression showed higher proliferation potential and lower apoptosis than differentiated cells. Conclusion: The colonies with stem cell properties indicated the existence of endometrial stem cell in rabbit endometrium
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