Toll样受体2/4在人牙龈上皮细胞的表达研究
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国家自然科学基金(81000444),江苏省自然科学基金(BK2008363)资助


Expression of toll-like receptor 2 and 4 in human gingival epithelial cells
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    摘要:

    目的:探讨Toll样受体2(toll-like receptor 2,TLR2)和Toll样受体4(toll-like receptor 4,TLR4)在人牙龈上皮细胞(human gingival epithelial cells,HGECs)的表达情况,以及上述受体在脂多糖(lipopolysaccharides,LPS)刺激下表达水平的改变-方法:采用逆转录PCR技术检测TLR2-TLR4 在HGECs的基因表达,采用real-time PCR技术和流式细胞技术检测1 μg/ml牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)-LPS或1 μg/ml大肠杆菌(Escherichia coli,E.coli) LPS刺激后,该细胞TLR2-TLR4表达水平的改变-结果:来自不同个体的HGECs均表达TLR2,TLR4 mRNA-1 μg/ml P.gingivalis LPS刺激后,TLR2基因和蛋白表达水平均明显增高(P < 0.05);1 μg/ml E.coli LPS刺激后,TLR4基因和蛋白表达水平明显增高(P < 0.05)-结论:TLR2-TLR4在HGECs存在稳定表达,并能被LPS的刺激活化,提示上述两种受体可能参与了牙周组织的炎症和免疫反应-

    Abstract:

    Objective: To observe the expression changes of toll-like receptor 2 and 4 (TLR2/4) in human gingival epithelial cells(HGECs) with and without lipopolysaccharides (LPS) treatment. Methods: TLR2/4 mRNA expressions were detected in HGECs by reverse transcription PCR. In addition,TLR2/4 mRNA and protein expressions were also determined in the cells stimulated with 1 μg/ml Porphyromonas gingivalis (P.gingivalis) LPS or 1 μg/ml Escherichia coli (E.coli) LPS by real-time PCR and FACS separately. Results: TLR2/4 mRNAs were expressed in HGECs from different individuals. Significant differences were found in TLR2 mRNA and protein expression in the cells with and without 1 μg/ml P.gingivalis LPS treatment(P < 0.05). In addition,significant differences were also found in TLR4 mRNA and protein expression in the cells with and without 1 μg/ml E.coli LPS treatment(P < 0.05). Conclusion: TLR2/4 were stably expressed in HGECs and their functions could be activated by LPS treatment,which may play a role in the development of periodontitis.

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李 慧,孙 颖,徐 艳,陈 武,狄昌萍. Toll样受体2/4在人牙龈上皮细胞的表达研究[J].南京医科大学学报(自然科学版),2011,(10):1483-1487

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  • 收稿日期:2011-03-16
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