文章摘要
刘 建,任宏轩,伍治平.RT-QPCR法检测早期NSCLC患者外周血中CK-19 mRNA的意义[J].南京医科大学学报,2011,(11):1640~1644
RT-QPCR法检测早期NSCLC患者外周血中CK-19 mRNA的意义
Using RT-QPCR to detect the expression of CK-19 mRNA in peripheral blood of NSCLC patients
投稿时间:2011-07-19  
DOI:10.7655
中文关键词: 非小细胞肺癌  微转移  细胞角蛋白-19  RT-PCR  ROC曲线
英文关键词: non-small cell lung cancer  micrometastases  CK-19  RT-PCR  receiver operating characteristic (ROC) curve
基金项目:云南省教育厅科研基金课题(08C0098)
作者单位
刘 建 昆明医学院第三附属医院,云南省肿瘤医院肿瘤化疗中心,云南 昆明 650118 
任宏轩 昆明医学院第三附属医院,云南省肿瘤医院肿瘤化疗中心,云南 昆明 650118 
伍治平 昆明医学院第三附属医院,云南省肿瘤医院肿瘤研究所,云南 昆明 650118 
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中文摘要:
      目的:RT-QPCR法探测早期非小细胞肺癌(NSCLC)外周血中CK-19 mRNA的意义?方法:以RT-PCR法从113例NSCLC患者(肺癌组)?50例肺良性病变和50例健康者(对照组)标本中筛选出有CK-19 mRNA表达的标本;再以RT-QPCR法检测表达CK-19 mRNA的标本,通过测定受试者标准曲线下面积(AUC)分析本实验的准确性?结果:①肺癌组和对照组中CK-19 mRNA阳性表达率分别为38.94%(44/113)?1.00%(1/100),差异有统计学意义(P < 0.01),且肺癌组中CK-19 mRNA的阳性表达与肿瘤分期和分化程度有关;②本实验效果的临界值为0.236,灵敏度为95.45%,准确度为77.27%;AUC为0.896?结论:RT-QPCR法检测外周血中的CK-19 mRNA可作为早期NSCLC患者发生微转移时的一个参考指标,但尚需大样本长期追踪观察证实?
英文摘要:
      Objective: To detect the expression of CK-19 mRNA in peripheral blood of non-small cell lung cancer(NSCLC) patients by real-time fluorescence quantitative polymerase chain reaction(RT-QPCR),and discuss the value of the experiment. Methods: The expression of CK-19 mRNA in peripheral blood of 113 NSCLC patients(NSCLC group),50 non-cancer patients and 50 healthy person(control group) was detected using RT- PCR and found out the positive cases. Then,RT-QPCR was used to detect the expression of relative levels of CK-19 mRNA in positive peripheral blood. The accuracy of this trial was analyzed by receiver operating characteristic curve(ROC curve) and the area under ROC curve(AUC). Results: ① The expression rates of CK-19 mRNA in the NSCLC group and the control group were 38.94%(44/113) and 1.00%(1/100),respectively,there was significant difference between them(P < 0.01),and the expression of CK-19 was related to TNM classification and differentiation of cancer. ② The analysis of ROC curve showed that when the point of transition was 0.236,its sensitivity and specificity were 95.45% and 77.27% respectively,and the AUC was 0.896. Conclusion: CK-19 mRNA might be the specific and sensitive marker to detect circulating tumor cells in peripheral blood of NSCLC patients,but it still needs to be proved by further follow-up observation in a large volume sample.
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