文章摘要
赵旺胜,王 芳,翁幸鐾,糜祖煌,潘世扬.基于管家基因和水平转移基因的菌株亲缘性分析:60株耐药大肠埃希菌研究[J].南京医科大学学报,2012,(1):109~114
基于管家基因和水平转移基因的菌株亲缘性分析:60株耐药大肠埃希菌研究
The relatedness in 60 drug-resistant Escherichia coli strains based on the analysis of the housekeeping genes and genes acquired by horizontal transfer
投稿时间:2011-07-15  
DOI:10.7655
中文关键词: 大肠埃希菌  菌株亲缘性
英文关键词: Escherichia coli  relatedness
基金项目:江苏省实验诊断学重点实验室重大项目(XK200731)
作者单位
赵旺胜 南京医科大学第一附属医院医学检验科,江苏 南京 210029 
王 芳 南京医科大学第一附属医院医学检验科,江苏 南京 210029 
翁幸鐾 宁波市第一医院检验科,浙江 宁波 315010 
糜祖煌 无锡市克隆遗传技术研究所,江苏 无锡 214026 
潘世扬 南京医科大学第一附属医院医学检验科,江苏 南京 210029 
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中文摘要:
      目的:了解1组(60株)耐药大肠埃希菌的菌株亲缘性?方法:对该组耐药大肠埃希菌4种与耐药相关的管家基因和45种水平转移获得与β-内酰胺类?氨基糖苷类?喹诺酮类耐药相关基因以及7种整合子?转座子?插入序列?接合性质粒遗传标记进行检测?并对检测结果进行样本聚类分析?结果:60株耐药大肠埃希菌多态性明显,多数菌株已发生演化?只有6?53号株;10?14号株,7?8?26?40号株3组携带相同基因(同一克隆)?结论:耐药基因的样本聚类分析可识别出具有相同耐药特征的菌株,具有一定的流行病学意义,为追溯耐药菌传播途径提供方便?
英文摘要:
      Objective: To investigate the relatedness in 60 drug-resistant Escherichia coli(E.coli) strains. Methods: Drug-resistance related genes were analyzed by PCR,including 4 kinds of housekeeping genes and 45 kinds of horizontal transfered genes related to 3 kinds of antimicrobial agents:beta-lactams,aminoglycosides,quinolones,as well as 7 kinds of genetic markers of integrons,transposons,insertion sequences and conjugal plasmids. They were used for the sample clustering analysis. Results: Polymorphism were distinct in 60 E.coli strains. Most strains showed evolution. Only 3 strain groups which consisted of strain 6 and 53,strain 10 and 14,strain 7,8,26 and 40,were the same clones carried the same genes. Conclusion: Sample clustering analysis of drug-resistance related genes could distinguish the strains with the same antimicrobial resistance characteristics,which was significant in epidemiologic research and provide convenience for tracing transmissible routes of drug-resistant strains.
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