文章摘要
李阿敏,崔学范,黄 茂.白介素-22诱导肺泡上皮2型人β防御素表达的初步研究[J].南京医科大学学报,2012,(2):199~203
白介素-22诱导肺泡上皮2型人β防御素表达的初步研究
Investigation of regulation of IL-22 on human β-defensin-2 expression in human alveolar epithelium
投稿时间:2011-05-31  
DOI:10.7655
中文关键词: 白介素-22  2型人β防御素  肺泡上皮细胞
英文关键词: interleukin-22  human β-defensin-2(HBD-2)  alveolar epithelial cells
基金项目:
作者单位
李阿敏 南京医科大学第一附属医院呼吸内科,江苏 南京 210029 
崔学范 南京医科大学第一附属医院呼吸内科,江苏 南京 210029 
黄 茂 南京医科大学第一附属医院呼吸内科,江苏 南京 210029 
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中文摘要:
      目的:初步探讨对于直接或间接引起的肺内感染,炎症反应产生的白介素(IL)-22能否刺激肺泡Ⅱ型上皮细胞,并且诱导其生成?释放2型人β防御素(human β-defensin-2,HBD-2)发挥抗感染作用?方法:流式细胞术检测肺泡Ⅱ型上皮细胞细胞膜表面IL-22R表达水平?重组人IL-22(20 ng/ml)刺激肺泡Ⅱ型上皮细胞,RT-PCR检测不同时间(0?2?4?8?16?24 h)HBD-2表达情况?不同浓度的重组人IL-22(0?4?20?100?500 ng/ml)刺激肺泡Ⅱ型上皮细胞,RT-PCR检测HBD-2表达情况?结果:肺泡Ⅱ型上皮细胞膜表面IL-22R表达呈强阳性,阳性率达(99.90 ± 0.13)%?比较6个时间点HBD-2表达差异,发现HBD-2表达具有时间依赖性?比较不同浓度IL-22处理下HBD-2表达差异,IL-22 100 ng/ml为最佳干预浓度,且HBD-2表达与IL-22浓度具有依赖关系?结论:IL-22能显著诱导肺泡上皮细胞生成并释放HBD-2,且具有时间和剂量依赖性?
英文摘要:
      Objective:To investigate whether human alveolar type Ⅱ epithelial cells express IL-22R and whether it generates human β-defensin-2 (HBD-2) under the stimulation with IL-22. Methods: First,flow cytometry(FCM) detected the expression of IL-22R on alveolar type Ⅱ epithelial cells;second,cells were stimulated with recombinant human IL-22(rhIL-22) for 0,2,4,8,16,24 hours,separately,and then RT-PCR tested the expression of HBD-2;third,the cells were stimulated with different concentration (0,4,20,100,500 ng/ml) of IL-22 for optimal dose. Results: The membranes of alveolar type Ⅱ epithelial cells highly expressed IL-22R and the positive rate is (99.90 ± 0.13)%;24 hours is an effective time for HBD-2 expression with IL-22 treatment,and the optimal concentration is 100 ng/ml. Conclusion: The alveolar type Ⅱ epithelial cells highly express IL-22R,and the interaction of IL-22 and IL-22R induces the HBD-2 gene expression in alveolar epithelium in a time- and concentration-dependent manner.
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