文章摘要
袁 昕,殷旭东,薛金俊,汪 瑞,张正荣,夏广鑫.食管鳞癌放疗前后外周血survivin检测的临床意义[J].南京医科大学学报,2012,(2):231~235
食管鳞癌放疗前后外周血survivin检测的临床意义
Clinical significances of survivin mRNA detection in peripheral blood of esophageal cancer patients treated with radiotherapy
投稿时间:2011-10-22  
DOI:10.7655
中文关键词: 食管鳞癌  放疗  生存素  预后  分子标记
英文关键词: esophagus squamous cell carcinoma  radiotherapy  survivin  prognosis  biomarker
基金项目:
作者单位
袁 昕 扬州市第一人民医院肿瘤科,江苏 扬州 225009 
殷旭东 扬州市第一人民医院肿瘤科,江苏 扬州 225009 
薛金俊 扬州市第一人民医院肿瘤科,江苏 扬州 225009 
汪 瑞 扬州市第一人民医院肿瘤科,江苏 扬州 225009 
张正荣 扬州市第一人民医院肿瘤科,江苏 扬州 225009 
夏广鑫 扬州市第一人民医院肿瘤科,江苏 扬州 225009 
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中文摘要:
      目的:研究食管鳞癌(esophagus squamous cell carcinoma,ESCC)患者放疗前后外周血循环肿瘤细胞(circulating tumor cells,CTCs)标记生存素(survivin) mRNA的表达及其临床意义?方法:收集72例ESCC患者根治性放疗前后外周血,应用巢式RT-PCR检测survivin mRNA的表达,分析其与临床病理特征?放疗疗效及两年无进展生存期(progerssion-free survival,PFS)的关系?结果:放疗前后survivin mRNA阳性者分别占54.2%(39/72)与38.9%(28/72),差异无统计学意义(P = 0.066),放疗前后survivin mRNA阳性均与淋巴结转移相关(P值分别为0.012和0.005),放疗后survivin mRNA阳性提示放疗疗效不佳(P = 0.030)?生存分析表明放疗前后survivin mRNA阳性与PFS减少有关 (P值分别为0.040和 < 0.001),Cox回归模型证实放疗后survivin mRNA阳性?KPS评分和规范化后续化疗是独立预后指标?结论:放疗后外周血survivin mRNA阳性有望成为ESCC根治性放疗疗效评估和预后判断的有效分子标记?
英文摘要:
      Objective: To study clinical significances of survivin expression,a marker of circulating tumor cells(CTC),in peripheral blood of esophagus squamous cell carcinoma(ESCC) patients treated with radiotherapy. Methods: Survivin mRNA expression was determined using nest RT-PCR in pre- and post-radiotherapy blood of 72 esophageal cancer patients with radiotherapy,and its associations with clinicopathological features,radiotherapy response and 2-year progression-free survival (PFS) were further evaluated. Results: The positive expression of survivin mRNA in pre- and post-radiotherapy were 54.2%(39/72) and 38.9%(28/72),respectively,and the difference has not statistical significance (P = 0.066). Furthermore,survivin mRNA expression was associated with lymph metastasis both in pre- and post-radiotherapy (P = 0.012 and P = 0.005,respectively). Survivin expression of post-radiotherapy was associated with poor radiotherapy response (P = 0.030). Kaplan-Meier analysis also showed that survivin mRNA expression predicted adverse 2-year PFS both in pre- and post-radiotherapy(P = 0.023 and P < 0.001). However,Cox proportional hazard models indicated that only post-radiotherapy survivin mRNA expression apart from KPS scores and standard chemotherapy were independent prognostic factors for ESCC. Conclusion: Post-radiotherapy survivin mRNA expression may be an useful biomarker related to poor radiotherapy response and adverse prognosis in ESCC patients treated with radiotherapy.
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