文章摘要
廖敏琪,傅 赞,于亚平,韩 涛.乳酸脱氢酶A在人胆管癌组织及胆管癌细胞中的表达及意义[J].南京医科大学学报,2012,(5):682~685
乳酸脱氢酶A在人胆管癌组织及胆管癌细胞中的表达及意义
Expression and significance of lactate dehydrogenase-A in human cholangiocarcinoma tissues and cell lines
投稿时间:2012-01-08  
DOI:10.7655
中文关键词: 胆管癌  乳酸脱氢酶A  有氧酵解
英文关键词: cholangiocarcinoma  lactate dehydrogenase-A  aerobic glycolysis
基金项目:江苏省兴卫工程重点人才专项基金(H201116)
作者单位
廖敏琪 南京医科大学第一附属医院普外科,江苏 南京 210029 
傅 赞 南京医科大学第一附属医院普外科,江苏 南京 210029 
于亚平 南京医科大学第一附属医院普外科,江苏 南京 210029 
韩 涛 南京医科大学第一附属医院普外科,江苏 南京 210029 
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中文摘要:
      目的:观察乳酸脱氢酶A(lactate dehydrogenase-A,LDH-A)在胆管癌组织及人胆管癌细胞株中的表达情况?方法:应用免疫组织化学方法检测32例胆管癌组织?15例癌旁组织中LDH-A的表达;采用反转录聚合酶链式反应(RT-PCR)?Western blot法检测LDH-A在人胆管癌细胞株HuCCT1及人正常胆管上皮细胞株HIBEpic中的表达?结果:LDH-A在胆管癌组织中呈高表达18/32(56.3%,P < 0.01),在HuCCT1细胞中亦呈现高表达,而HIBEpic细胞则表现为低表达?结论:LDH-A在胆管癌组织及人胆管癌细胞株HuCCT1中高表达,说明LDH-A与胆管癌的发生?发展可能具有关联性?
英文摘要:
      Objective:To observe the expression of lactate dehydrogenase-A(LDH-A) in cholangiocarcinoma tissues,human cholangiocarcinoma cell line HuCCT1 and human normal bile duct epithelium cell line HIBEpic. Methods: The expression of LDH-A in 32 cholangiocarcinoma and 15 adjacent non-cancerous tissues was analyzed by immunohistochemistry. RT-PCR and Western blotting assays were used to detect the expression of LDH-A in human cholangiocarcinoma cell line HuCCT1 and human normal bile duct epithelium cell line HIBEpic. Results: LDH-A was overexpressed in 56.3% (18/32) cholangiocarcinoma tissues (P < 0.01) and in HuCCT1 cells but not in HIBEpic cells. Conclusion: Cholangiocarcinoma tissues and human cholangiocarcinoma cell lines HuCCT1 overexpress LDH-A,indicating that LDH-A may play an important role in the initiation and progression of cholangiocarcinoma.
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