文章摘要
吴冬鸣,唐 燕,韩 艺,白文莉,谢利平,刘 振,孟国梁,季 勇.大鼠心肌缺血再灌注后不同时间点心脏及肝脏中HIF-1、VEGF的表达[J].南京医科大学学报,2012,(8):1047~1051
大鼠心肌缺血再灌注后不同时间点心脏及肝脏中HIF-1、VEGF的表达
The expression of HIF-1 and VEGF in myocardium and liver after myocardial ischemia reperfusion injury
投稿时间:2012-04-18  
DOI:10.7655
中文关键词: 心肌  缺血再灌注  HIF-1  VEGF
英文关键词: myocardium  ischemia reperfusion  HIF-1  VEGF
基金项目:国家自然科学基金面上项目(30971256);江苏省高校自然科学研究重大项目(10KJA310033)
作者单位
吴冬鸣 淮安市第四人民医院肝病科,江苏 淮安 223002 
唐 燕 南京医科大学病理生理学系,动脉粥样硬化中心,江苏省心血管病分子干预重点实验室,江苏 南京 210029 
韩 艺 南京医科大学第一附属医院老年科,江苏 南京 210029 
白文莉 南京医科大学病理生理学系,动脉粥样硬化中心,江苏省心血管病分子干预重点实验室,江苏 南京 210029 
谢利平 南京医科大学病理生理学系,动脉粥样硬化中心,江苏省心血管病分子干预重点实验室,江苏 南京 210029 
刘 振 南京医科大学病理生理学系,动脉粥样硬化中心,江苏省心血管病分子干预重点实验室,江苏 南京 210029 
孟国梁 南京医科大学病理生理学系,动脉粥样硬化中心,江苏省心血管病分子干预重点实验室,江苏 南京 210029 
季 勇 南京医科大学病理生理学系,动脉粥样硬化中心,江苏省心血管病分子干预重点实验室,江苏 南京 210029 
摘要点击次数: 1241
全文下载次数: 946
中文摘要:
      目的:探讨大鼠心肌缺血再灌注后不同时间点心脏和肝脏中缺氧诱导因子-1(hypoxia-inducible factor 1,HIF-1)及血管内皮生长因子(vascular endothelial growth factor,VEGF)的变化?方法:结扎雄性成年SD大鼠冠状动脉左前降支,缺血30 min,再灌注6 h(I30minR6h)或24 h(I30minR24h),real-time PCR法检测心肌组织中HIF-1α和VEGF的mRNA水平,Western blot法检测心肌以及肝脏组织中HIF-1α?HIF-1β 和VEGF蛋白表达的改变?结果:与假手术组相比,I30minR6h组心肌中 HIF-1α和VEGF mRNA和蛋白水平明显增加,但I30minR24h组无明显变化?心肌组织中HIF-1β蛋白水平及肝脏中HIF-1α?HIF-1β 和VEGF蛋白水平在不同再灌注时间点无明显改变?结论:心肌缺血再灌注影响心肌HIF-1和VEGF的表达,但对肝脏中HIF-1和VEGF的表达无影响,且其对心肌HIF-1和VEGF表达的影响与再灌注时间相关?
英文摘要:
      Objective:To study the expression of hypoxia-inducible factor 1(HIF-1) and vascular endothelial growth factor(VEGF) in myocardium and liver after myocardial ischemia reperfusion injury. Methods:Adult male SD rats were subjected to 30 min myocardial ischemia by ligation of the left anterior descending coronary artery and 6 h (I30minR6h) or 24h (I30minR24h) reperfusion. The protein expression of HIF-1α,HIF-1β and VEGF in myocardium and liver were examined by Western blot,and the mRNA levels of HIF-1α and VEGF were determined by real-time PCR. Results:Compared with sham group,both protein and mRNA levels of HIF-1α and VEGF were increased in the ischemic region of myocardium in I30minR6h,but unaffected in I30minR24h. The level of HIF-1β was not different in myocardium among the three groups. But compared with sham group,there were no differences of the protein expression of HIF-1α,HIF-1β or VEGF in liver after myocardial I30minR6h or I30minR24h. Conclusion:The expression of HIF-1α and VEGF in myocardium were affected in myocardial ischemia reperfusion injury whereas not changed in liver. The affection was associated with the time of reperfusion.
查看全文   查看/发表评论  下载PDF阅读器