文章摘要
张 春,茌 静,顾 斌,郭丹丹,张国锋,周 锋,魏 栋,姚 堃,胡卫星.人疱疹病毒6型感染与神经胶质瘤关系的初步研究[J].南京医科大学学报,2012,(8):1052~1056
人疱疹病毒6型感染与神经胶质瘤关系的初步研究
Preliminary study on the relationship between human herpesvirus-6 infection and glioma
投稿时间:2010-08-29  
DOI:10.7655
中文关键词: 人疱疹病毒6型  神经胶质瘤  巢式PCR  免疫组化
英文关键词: human herpesvirus-6  glioma  nested PCR  immunohistochemistry
基金项目:国家自然科学基金资助(30972784);江苏省医学重点人才工程(135工程)资助(RC2002075)
作者单位
张 春 南京医科大学第一附属医院神经外科,江苏 南京 210029 
茌 静 南京医科大学微生物与免疫学系,江苏 南京 210029 
顾 斌 南京医科大学第一附属医院神经外科,江苏 南京 210029 
郭丹丹 南京医科大学微生物与免疫学系,江苏 南京 210029 
张国锋 南京医科大学第一附属医院神经外科,江苏 南京 210029 
周 锋 南京医科大学微生物与免疫学系,江苏 南京 210029 
魏 栋 南京医科大学第一附属医院神经外科,江苏 南京 210029 
姚 堃 南京医科大学微生物与免疫学系,江苏 南京 210029 
胡卫星 南京医科大学第一附属医院神经外科,江苏 南京 210029 
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中文摘要:
      目的:研究人疱疹病毒6型(human herpesvirus-6,HHV-6)感染与神经胶质瘤的关系?方法:巢式PCR法检测40例神经胶质瘤样本和13例正常脑组织样本中HHV-6?人巨细胞病毒(human cytomegalovirus,HCMV)?HHV-7 DNA序列?免疫组化染色法检测神经胶质瘤样本和正常脑组织样本中HHV-6?HCMV?HHV-7抗原的表达?结果:巢式PCR法检测结果显示,神经胶质瘤组织HHV-6 DNA阳性率为42.5%,正常脑组织HHV-6 DNA阳性率为7.7%(P = 0.020),神经胶质瘤组织HCMV?HHV-7 DNA阳性率分别为20.0%和5.0%,正常脑组织未检测到HCMV和HHV-7 DNA(P值分别为0.087和0.566)?进一步用免疫组化染色法检测HHV-6早期抗原p41的表达,神经胶质瘤组织阳性率为27.5%,正常脑组织未见阳性表达(P = 0.030)?同时检测HHV-6晚期抗原gp116/64/54的表达,神经胶质瘤组织阳性率为32.5%,正常脑组织未见阳性表达(P = 0.014)?用HCMV pp65抗体进行免疫组化染色后发现其在神经胶质瘤中阳性率为12.5%,正常脑组织未见阳性表达(P = 0.229)?HHV-7 pp85抗原在神经胶质瘤样本和正常脑组织样本中均未检出阳性表达?结论:根据PCR和免疫组化的检测结果,HHV-6感染在神经胶质瘤和正常脑组织中具有显著性差异,HHV-6感染在神经胶质瘤的病因和发展过程中起了一定作用?
英文摘要:
      Objective: To study the relationship between human herpesvirus(HHV)-6 infection and glioma. Methods:HHV-6, human cytomegalovirus(HCMV) and HHV-7 DNA in glioma samples(n = 40) and control samples(n = 13) were detected by nested PCR. The expression of HHV-6, HCMV and HHV-7 was detected by immunohistochemistry(IHC). Results:HHV-6 DNA was positive in 17/40(42.5%) of glioma samples and 1/13(7.7%) of normal brain samples(P = 0.020), while the positive rates of HCMV and HHV-7 DNA were 20.0% and 5.0% in glioma,respectively. HCMV and HHV-7 DNA hadn’t been found in normal brain samples(P = 0.087 and 0.566 respectively). HHV-6 late antigen p41 was expressed in 11/40(27.5%) of tumors and 0 of the controls(P = 0.030), while HHV-6 early antigen gp116/64/54 was expressed in 13/40(32.5%) of tumors and 0 of the controls(P = 0.014). HCMV pp65 antigen was expressed in 5/40(12.5%) of glioma samples and 0/13 of the normal brain controls(P = 0.229). HHV-7 pp85 antigen hadn’t been found in tumors and normal brain samples. Conclusion:Based on the results of nested PCR and immunohistochemistry,significant difference of HHV-6 infection between glioma and normal brain tissue was confirmed. HHV-6 infection may play a role on the pathagenesis and progression of glioma.
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