文章摘要
季润元,史恩溢,王永实,李小宁,赵玉芳.应用组织芯片技术检测结直肠癌中MRP1/CD9的表达[J].南京医科大学学报,2012,(8):1066~1069
应用组织芯片技术检测结直肠癌中MRP1/CD9的表达
Detecting of MRP1/CD9 in colorectal carcinoma using tissue microarrays
投稿时间:2012-03-31  
DOI:10.7655
中文关键词: 结直肠癌  MRP1/CD9  转移  组织芯片  免疫组织化学
英文关键词: colorectal carcinoma  MRP1/CD9  metastasis  tissue microarray  immunohistochemistry
基金项目:江苏省卫生厅医学科技发展基金(J201116)
作者单位
季润元 淮阴卫生高等职业技术学校病理教研室,江苏 淮安 223300 
史恩溢 苏州卫生职业技术学院病理教研室,江苏 苏州 215009 
王永实 淮阴卫生高等职业技术学校病理教研室,江苏 淮安 223300 
李小宁 苏州卫生职业技术学院病理教研室,江苏 苏州 215009 
赵玉芳 淮安市肿瘤医院病理科,江苏 淮安 223200 
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中文摘要:
      目的:探讨结直肠癌组织中运动相关蛋白-1(MRP1/CD9)的表达及其与肿瘤转移的关系?方法:应用组织芯片和免疫组织化学技术检测244例结直肠癌组织中MRP1/CD9的蛋白表达?结果:MRP1/CD9在结直肠癌组织中阳性率为77.9%,其表达与患者的性别?年龄?浸润深度无关,而与肿瘤的分化程度?Dukes分期?淋巴结转移有关;其中高-中分化组表达高于低分化组;Dukes A+B期表达高于C+D组;无淋巴结转移组表达高于有淋巴转移组,差异均具有统计学意义(P < 0.05)?二元Logistic回归前进法显示:分化程度最先进入方程,是影响MRP1/CD9表达最显著因素?MRP1/CD9表达在单因素时和Dukes分期负相关,但在多因素时被掩盖?结论:MRP1/CD9在结直肠癌组织中的表达缺失与肿瘤的转移密切相关,有望成为判断结直肠癌预后的新标志?
英文摘要:
      Objective:To investigate the expression of MRP1/CD9 as well as their correlations with the metastasis of colorectal carcinoma. Methods:Tissue microarray and immunohistochemistry were used to detect the expression of MRP1/CD9 in 244 cases of colorectal carcinoma. Results:The positive rate of MRP1/CD9 expression was 77.9% in colorectal carcinoma. The expression of MRP1/CD9 had no relationship with the patients’ sex,age,and the depth of infiltration,but it had relationships with the differentiated degree,Dukes stages,and lymphnode metastasis. The expression of MRP1/CD9 was higher in colorectal carcinomas with moderate or high differention than that in the ones with poor differention. The expression of MRP1/CD9 was higher in colorectal carcinomas with Dukes A+B stages than that in the ones with Dukes C+D stages. The expression of MRP1/CD9 was higher in colorectal carcinomas without lymphnode metastasis than that in the ones with lymphnode metastasis. Above all had the significant meaning(P < 0.05). Forward stepwise binary logistic regression reveals:differentiation,which went into the equation first,is the significant factor of MRP1/CD9 expression. The expression of MRP1/CD9 was negatively related to Dukes stages in monofactoral analysis,but disappearing in multifactoral analysis. Conclusion:The loss expression of MRP1/CD9 are closed correlated with the metastasis of colorectal carcinoma,and this marker has hopes to become a new target to evaluate the tumor prognosis.
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